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Molecular and General Genetics MGG

, Volume 109, Issue 4, pp 323–337 | Cite as

The molecular structure of the transducing particles ofSalmonella phageP22

II. Density gradient analysis of DNA
  • Horst Schmiteger
Article

Summary

CsCl density gradient analysis showed that the DNA of plaque forming particles ofSalmonella phageP22 is lighter than the host DNA. The DNA of transducing phages exhibits an intermediate density, but close to host DNA. BU labelling of DNA synthesized in the cells after phage infection resulted in a density increase of transducing DNA of about 0.004 gxcm-3, whereas infectious DNA increased by about 0.045 gxcm-3. Shearing of isolated DNA molecules from unlabelledP22 lysates demonstrated that transducing DNA consists of two pieces of DNA of different density: 90% stem from the bacterial host whereas 10% are phage DNA and therefore responsible for the BU lable in transducing phages.

Keywords

Molecular Structure Density Gradient Density Increase CsCl Gradient Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bostein, D.: Synthesis and maturation of phageP22 DNA. I. Identification of intermediates. J. molec. Biol.34, 621–641 (1968).CrossRefGoogle Scholar
  2. Britten, R. J., Roberts, R. B.: High-resolution density gradient sedimentation analysis. Science131, 32–33 (1960).CrossRefGoogle Scholar
  3. Burgi, E., Hershey, A. D.: Sedimentation rate as a measure of molecular weight of DNA. Biophys. J.3, 309–321 (1963).CrossRefGoogle Scholar
  4. Chan, H., Lark, K. G.: Chromosome replication inSalmonella typhimurium. J. Bact.97, 848–860 (1969).PubMedGoogle Scholar
  5. Flamm, W. G., Bond, H. E., Burr, H. E.: Density-gradient centrifugation of DNA in a fixedangle rotor. A higher order of resolution. Biochim. biophys. Acta (Amst.)129, 310–319 (1966).CrossRefGoogle Scholar
  6. Ikeda, H., Tomizawa, J.: Transducing fragments in generalized transduction by phageP1. I. Molecular origin of the fragments. J. molec. Biol.14, 85–109 (1965a).CrossRefGoogle Scholar
  7. — Transducing fragments in generalized transduction by phageP1. II. Association of DNA and protein in the fragments. J. molec. Biol.14, 110–119 (1965b).CrossRefGoogle Scholar
  8. Kozinski, A. W., Szybalski, W.: Dispersive transfer of the parental DNA molecule to the progeny of phage øX-174. Virology9, 260–274 (1959).CrossRefGoogle Scholar
  9. Nishioka, Y.: Sequence of genes replicated inSalmonella typhimurium as examined by transduction techniques. Kansas State University, Thesis 1969.Google Scholar
  10. Sanderson, K. E., Demerec, M.: The linkage map ofSalmonella typhimurium. Genetics51, 897–913 (1965).PubMedPubMedCentralGoogle Scholar
  11. Schmieger, H.: Die molekulare Struktur transduzierender Partikel beimSalmonella-PhagenP22. I. Dichtegradienten-Untersuchungen an intakten Phagen. Molec. Gen. Genetics101, 336–347 (1968).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Horst Schmiteger
    • 1
  1. 1.Abteilung Mikrobengenetik GöttingenInstitut für Mikrobiologie der Gesellschaft für Strahlenforschung mbH, MünchenGermany

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