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Thirteen phenotypic revertants from streptomycin dependence were independently isolated. The ribosomal proteins from these mutants were extracted and analyzed by two-dimensional polyacrylamide gel electrophoresis and by immunological methods. In four of these mutants examined, alterations in protein S4 were detected by a change in electrophoretic mobility. Three additional mutants have S4 proteins which are indistinguishable from the wild type in two-dimensional electrophoresis but which are clearly different in their immunological properties. Further analysis should reveal whether suppression of the streptomycin independent phenotype is always caused by an alteration in protein S4.
KeywordsElectrophoresis Polyacrylamide Streptomycin Ribosomal Protein Electrophoretic Mobility
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