Post-microsomal supernatant enzymes isolated from adults of the Abnormal Abdomen (A 53g) mutant strain of Drosophila melanogaster stimulate the in vitro aminoacylation of tRNA. This stimulation is due to an altered enzyme system in the mutant which exhibits an increased level of aminoacylation of isoaccepting tRNA molecules. Observations of in vitro aminoacylation studies, as well as the results of MAK and BD-cellulose column chromatography of tRNAs aminoacylated by mutant and wild-type Drosophila supernatant enzymes, support this hypothesis.
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This research was supported in part by an institutional grant IN 88C from the American Cancer Society and in part by Grant Number GM 18080 from the United States Public Health Service. A portion of this research was presented by R. W. R. in a dissertation to the Department of Biology, Temple University, in partial fulfillment of the requirements for the Ph. D.
Communicated by H. G. Wittmann
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Rose, R.W., Hillman, R. Increased aminoacylation of tRNA as a function of the Abnormal abdomen genotype of Drosophila melanogaster . Molec. Gen. Genet. 133, 213–224 (1974). https://doi.org/10.1007/BF00267671
- Column Chromatography
- Mutant Strain
- Enzyme System