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Increased aminoacylation of tRNA as a function of the Abnormal abdomen genotype of Drosophila melanogaster


Post-microsomal supernatant enzymes isolated from adults of the Abnormal Abdomen (A 53g) mutant strain of Drosophila melanogaster stimulate the in vitro aminoacylation of tRNA. This stimulation is due to an altered enzyme system in the mutant which exhibits an increased level of aminoacylation of isoaccepting tRNA molecules. Observations of in vitro aminoacylation studies, as well as the results of MAK and BD-cellulose column chromatography of tRNAs aminoacylated by mutant and wild-type Drosophila supernatant enzymes, support this hypothesis.

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  1. Colowick, S. P., Kaplan, N. O.: In: Method in Enzymology (L. Grossman, K. Moldave, eds.) vol. XII, Nucleic Acids, part B, p. 87. New York: Academic Press 1968

  2. Doolittle, W. F., Yanofsky, C.: Mutants of E. coli with an altered tryptophanyl-transfer ribonucleic acid synthetase. J. Bact. 95, 1283 (1968)

  3. Fox, A. S., Kan, J., Kang, S. H., Wallis, B.: Protein synthesis in cell-free preparations from Drosophila melanogaster. J. biol. Chem. 240, 2059 (1965)

  4. Gillam, I., Millward, S., Blew, D., von Tigerstrom, M., Wimmer, E., Tener, G. M.: The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry 6, 3043 (1967)

  5. Hartwell, L. H., McLaughlin, C. S.: Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases. Proc. nat. Acad. Sci. (Wash.) 59, 422 (1968)

  6. Hirschfield, I. N., Bloemers, H. P. J.: The biochemical characterization of two mutant arginyl tRNA synthetases from E. coli K-12. J. biol. Chem. 244, 2911 (1969)

  7. Ilan, J.: Amino acid incorporation and aminoacyl transfer in an insect pupal system. J. biol. Chem. 243, 5859 (1968)

  8. Ilan, J., Lipmann, F.: A cell-free protein synthesis system from pupae of Tenebrio molitor. Acta biochem. pol. 13, 353 (1966)

  9. Jenny, E., Hicklin, A., Leuthardt, F.: In vitro Einbau radioaktiver Aminosäuren in die Proteine von Drosophila Puppen. Helv. chim. Acta 45, 2014 (1962)

  10. Litvak, S., Mancilla, J., Agosin, M.: Protein synthesis in cell-free preparations from Musca domestica L. Comp. Biochem. Physiol. 21, 441 (1967)

  11. Loftfield, R. B.: The aminoacylation of transfer ribonucleic acid. In: Protein Synthesis (E. H. McConkey, ed.), vol. 1., p. 1. New York: Marcel Dekker 1971

  12. Mandell, J. D., Hershey, A. D.: A fractionating column for analysis of nucleic acids. Analyt. Biochem. 1, 66 (1960)

  13. McLaughlin, C. S., Hartwell, L. H.: A mutant of yeast with a defective methionyl-tRNA synthetase. Genetics 61, 557 (1969)

  14. Morrison, W. W., Frajola W. J.: The stimulation of tryptophan pyrrolase activity by RNA fractions isolated at various temperatures. Biochem. biophys. Res. Commun. 17, 597 (1964)

  15. Printz, D. B., Gross, S. R.: An apparent relationship between mistranslation and an altered leucyl-tRNA synthetase in a conditional lethal mutant of Neurospora crassa. Genetics 55, 451 (1967)

  16. Rose, R., Hillman, R.: In vitro studies of protein synthesis in Drosophila. Biochem. biophys. Res. Commun. 35, 197 (1969)

  17. Sekeri, K. E., Sekeris, C. E., Karlson, P.: Protein synthesis in subcellular fractions of the blowfly during different developmental stages. J. Insect Physiol. 14, 425 (1968)

  18. Sueoka, N., Yamane, T.: Fractionation of aminoacyl-acceptor RNA on a methylated albumin column. Proc. nat. Acad. Sci. (Wash.) 48, 1454 (1962)

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This research was supported in part by an institutional grant IN 88C from the American Cancer Society and in part by Grant Number GM 18080 from the United States Public Health Service. A portion of this research was presented by R. W. R. in a dissertation to the Department of Biology, Temple University, in partial fulfillment of the requirements for the Ph. D.

Communicated by H. G. Wittmann

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Rose, R.W., Hillman, R. Increased aminoacylation of tRNA as a function of the Abnormal abdomen genotype of Drosophila melanogaster . Molec. Gen. Genet. 133, 213–224 (1974).

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  • Enzyme
  • Chromatography
  • Column Chromatography
  • Mutant Strain
  • Enzyme System