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Rapid clonal multiplication of Digitalis lanata in tissue culture

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Abstract

Shoot cultures of Digitalis lanata have been established by inoculating the shoot tip of seedlings germinated in aseptic culture, or of field-grown plants, onto Linsmaier and Skoog's RM medium supplemented with 1 mg 6-benzylaminopurine and 0.1 mg indolacetic acid 1−1. On this medium formation of up to 30 new axillary shoots could be induced. Shoots could be grown into functional plants after root induction on a medium containing reduced amounts (one-fifth of normal) of nitrogen and indolebutyric acid (0.5 mg 1−1).

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References

  1. Linsmaier EM, Skoog F (1965) Physiol Plant 18: 100–127

  2. Reinhard E, Alfermann AW (1980) in: Fiechter A (ed) Plant Cell Cultures. Adv Biochem Engin 16: 50–83

  3. Vasil IK, Vasil V (1980) in: Vasil IK (ed) Perspectives in plant cell and tissue cultures. Int Rev Cytol Suppl 11A: 145–163

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Erdei, I., Kiss, Z. & Maliga, P. Rapid clonal multiplication of Digitalis lanata in tissue culture. Plant Cell Reports 1, 34–35 (1981). https://doi.org/10.1007/BF00267655

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Keywords

  • Nitrogen
  • Germinate
  • Tissue Culture
  • Clonal Multiplication
  • Root Induction