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Polar uncoating of tobacco mosaic virus (TMV) with dimethylsulfoxide (DMSO) and subsequent reassembly of partially stripped TMV

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Summary

Increasing concentrations of dimethylsulfoxide (DMSO) strip tobacco mosaic virus (TMV) stepwise from the 3′ end. The RNA tail increases in length up to 2,000 nucleotides (nu) reaching a region of very strong protein-RNA affinity. Thereafter, uncoating occurs from the other end and produces a second RNA tail 500 nu long. Further stripping of TMV proceeds from both ends, the long tail increasing in length up to 4,000 nu and the short one increasing more moderately and remaining below 2,000 nu. The region of strongest protein-RNA affinity is located between 4,000 and 5,000 nu away from the 3′ end.

Using the same conditions as for in vitro TMV reassembly, it is possible to recoat the RNA tails with viral protein preferentially in the 5′ direction.

The advantages of DMSO in studies of TMV protein-RNA interactions are discussed.

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References

  1. Buzzel, A.: Action of urea on tobacco mosaic virus. J. Am. Chem. Soc. 82, 1636–1641 (1960)

  2. Caspar, D.L.D.: Assembly and stability of the tobacco mosaic virus particle. Adv. Protein Chem. 18, 37–21 (1963)

  3. Harrington, W.F., Schachman, H.K.: Studies on the alkaline degradation of tobacco mosaic virus. I. Ultracentrifugal analysis. Arch. Biochem. Biophys 65, 278–295 (1956)

  4. Hunter, T.R., Hunt, T., Knowland, J., Zimmern, D.: Messenger RNA for the coat protein of tobacco mosaic virus. Nature 260, 759–764 (1976)

  5. Lebeurier, G., Hirth, L.: TMV reconstitution at low ionic strength. FEBS Letters 34, 19–23 (1973)

  6. Lebeurier, G., Nicolaïeff, A., Richards, K.E.: Inside-out model for self assembly of tobacco mosaic virus. Proc. Natl. Acad. Sci. U.S.A. 74, 149–153 (1977)

  7. Lindley, I.J.D., Stebbing, N.: Aminoacylation of encephalomyocarditis virus RNA. J. Gen. Virol. 34, 177–182 (1977)

  8. Longchampt, M., Lebeurier, G., Hirth, L.: Polymerization of tobacco mosaic virus protein in Na pyrophosphate. FEBS Letters 22, 297–300 (1972)

  9. May, D.S., Knight, C.A.: Polar stripping of protein subunits from tobacco mosaic virus. Virology 25, 502–507 (1965)

  10. Nicolaïeff, A.: Electron microscopy of single stranded RNA: spreading with dimethylsulfoxide (DMSO). (Submitted)

  11. Nicolaïeff, A., Lebeurier, G., Morel, M.C., Hirth, L.: The uncoating of native and reconstituted TMV by DMSO: the polarity of stripping. J. Gen. Virol. 26, 295–306 (1975)

  12. Onda, H., Taniguchi, T., Matsui, C.: Polar stripping of tobacco mosaic virus by alkali. Virology 42, 551–553 (1970).

  13. Perham, R.N.: Self-assembly of biological macromolecules. Philos. Trans. R. Soc.Lond. [Biol.] 272, 123–136 (1975)

  14. Perham, R.N., Wilson, T.M.A.: The polarity of stripping of coat protein subunits from the RNa in tobacco mosaic virus under alkaline conditions. FEBS Letters 62, 11–15 (1976)

  15. Perham, R.N., Wilson, T.M.A.: The characterization of intermediates formed during the disassembly of tobacco mosaic virus at alkaline pH. Virology 84, 293–302 (1978)

  16. Richards, K.E., Morel, M.C., Nicolaïeff, A., Lebeurier, G., Hirth, L.: Location of the cistron of the tobacco mosaic virus coat protein. Biochimie 57, 749–755 (1975)

  17. Schramm, G., Schumacher, G., Zillig, W.: Über die Struktur der Tabakmosaikvirus. III. Der Zerfall in alkalischer Lösung. Z. Naturforsch. 10b, 481–492 (1955)

  18. Salomon, R., Littauer, U.Z.: Enzymatic acylation of histidine to mengovirus RNA. Nature 249, 32–34 (1974)

  19. Symington, J.: Linear variation in stability of tobacco mosaic virus to stripping of protein by detergent. Virology 38, 309–316 (1969)

  20. Wilson, T.M.A.: The polarity of stripping of coat protein subunit from the RNA in tobacco mosaic virus by dimethylsulfoxide FEBS Letters 87, 17–20 (1978)

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Correspondence to Anatole Nicolaïeff.

Additional information

Communicated by H.G. Wittmann

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Nicolaïeff, A., Lebeurier, G. Polar uncoating of tobacco mosaic virus (TMV) with dimethylsulfoxide (DMSO) and subsequent reassembly of partially stripped TMV. Molec. Gen. Genet. 171, 327–333 (1979). https://doi.org/10.1007/BF00267588

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Keywords

  • Nucleotide
  • DMSO
  • Dimethylsulfoxide
  • Mosaic Virus
  • Viral Protein