During transformation of B. subtilis cells with the Bsu R restriction-modification system by means of pUB110 plasmid restriction and modification of the plasmid DNA occurs. The effect of restriction on the transformation frequency is relatively weak, bringing about 20-fold decrease only. When using cells of a modifying recipient, the frequency of AR9 phage-mediated transduction of unmodified plasmid DNA is also relatively little decreased. The frequency of transduction by chromosomal markers, under the same conditions, falls much lower.
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Communicated by H. Böhme
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Prozorov, A.A., Belova, T.S. & Surikov, N.N. Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid. Molec. Gen. Genet. 180, 135–138 (1980). https://doi.org/10.1007/BF00267362
- Bacillus Subtilis
- Transformation Frequency
- Chromosomal Marker
- Subtilis Strain