Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid

  • 58 Accesses

  • 6 Citations

Summary

During transformation of B. subtilis cells with the Bsu R restriction-modification system by means of pUB110 plasmid restriction and modification of the plasmid DNA occurs. The effect of restriction on the transformation frequency is relatively weak, bringing about 20-fold decrease only. When using cells of a modifying recipient, the frequency of AR9 phage-mediated transduction of unmodified plasmid DNA is also relatively little decreased. The frequency of transduction by chromosomal markers, under the same conditions, falls much lower.

This is a preview of subscription content, log in to check access.

References

  1. Contente S, Dubnau D (1979) Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation. Mol Gen Genet 167:251–258

  2. Ferrari E, Canosi U, Galizzi A, Mazza G (1978) Studies on transduction process by SPP1 phage. J Gen Virol 41:563–572

  3. Gryczan T, Contente S, Dubnau D (1978) Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol 134:318–329

  4. Ikawa S, Shibata T, Ando T, Saito H (1979) Host-controlled modification and restriction in Bacillus subtilis. Bsu 168-system in Bacillus subtilis. Mol Gen Genet 170:123–127

  5. Makino O, Kawamura F, Saito H, Ikeda J (1979) Inactivation of restriction endonuclease Bam NX after infection with phage ϑ NR2. Nature 277:64–66

  6. Tanaka T (1979) Restriction of plasmid-mediated transformation in Bacillus subtilis 168. Mol Gen Genet 175:235–238

  7. Trautner T, Pawlek B, Bron S, Anagnostopoulos C (1974) Restriction and modification in Bacillus subtilis. Biological aspects. Mol Gen Genet 131:181–191

Download references

Author information

Correspondence to Alexandr A. Prozorov.

Additional information

Communicated by H. Böhme

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Prozorov, A.A., Belova, T.S. & Surikov, N.N. Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid. Molec. Gen. Genet. 180, 135–138 (1980). https://doi.org/10.1007/BF00267362

Download citation

Keywords

  • Bacillus
  • Bacillus Subtilis
  • Transformation Frequency
  • Chromosomal Marker
  • Subtilis Strain