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Identification of the structural gene of levansucrase in Bacillus subtilis Marburg


Two mutations affecting either the temperature sensitivity or the catalytic properties of the levansucrase of Bacillus subtilis Marburg were characterized. The relevant altered levansucrases were purified and their properties compared with those of the unmodified levansucrase. The results of this analysis and the genetic mapping of these mutations indicates that the structural gene of levansucrase is located in the sacB locus on the Bacillus subtilis chromosome between the cysB3 and hisA1 markers.

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Communicated by E. Bautz

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Lepesant, J.-., Lepesant-Kejzlarová, J., Pascal, M. et al. Identification of the structural gene of levansucrase in Bacillus subtilis Marburg. Molec. Gen. Genet. 128, 213–221 (1974).

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  • Bacillus
  • Structural Gene
  • Genetic Mapping
  • Bacillus Subtilis
  • Catalytic Property