The plasmid pMY3, which was constructed so as to express the Su+7 amber suppressor tRNA gene, also relaxes control of stable RNA synthesis in stringent cells. The relaxation is not growth medium or strain-dependent and does not occur in the presence of the vehicle alone. When expression of the effective sequence is diminished, in a lysogen of ϕ80d3ilv+Su+7, the sequence no longer affects RNA synthesis.
The relaxation is general, extending to all or almost all tRNA loci, including tRNAs located in the ribosomal spacer regions, and to all ribosomal RNAs. Relaxed plasmid-carrying strains are still able to elevate guanosine tetra- and penta-phosphate levels in response to amino acid starvation, but steady state levels are somewhat diminished. Aminoacyl-tRNA falls to control levels when the plasmid-carrying strain is deprived of amino acid. Therefore, the relaxed strain perceives amino acid starvation, but does not respond normally. These properties define a novel locus which relaxes stringent control.
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Bachman, B.G., Low, J.B., Taylor, A.L.: Recalibrated linkage map of E. coli. Bacteriol. Rev. 40, 116–167 (1976)
Block, R., Haseltine, W.A.: Thermolability of the stringent factor in rel mutants of E. coli. J. Mol. Biol. 77, 625–629 (1973)
Block, R., Haseltine, W.A.: In vitro synthesis of ppGpp and pppGpp. In: Ribosomes (M. Nomura, A. Tissieres and P. Langyel, eds.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1974
Bonner, W.M., Laskey, R.A.: A film detection method for tritiumlabeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46, 83–88 (1974)
Cashel, M., Gallant, J.: Two compounds implicated in the function of the RC gene of E. coli. Nature 221, 838–841 (1969)
Cashel, M., Lazzarini, R.A., Kalbacher, B.: An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labeled orthophosphate. J. Chromatogr. 40, 103–109 (1969)
Edlin, G., Neuhard, J.: Regulation of nucleoside triphosphate pools in E. coli. J. Mol. Biol. 24, 225–230 (1967)
Fiil, N.P., Meyenburg, K. von, Friesen, J.D.: Accumulation and turnover of guanosine tetraphosphate in E. coli. J. Mol. Biol. 71, 769–783 (1972)
Fiil, N.P., Williamsen, B.M., Friesen, J.D., Meyenburg, K. von: Interaction of alleles of the relA, relC, and spoT genes in E. coli: Analysis of the interconversion of GTP, ppGpp and pppGpp. Mol. Gen. Genet. 150, 87–101 (1977)
Folk, W.R., Berg, P.: Characterization of altered forms of glycyltransfer RNA synthetase and the effects of such alterations on aminoacyl tRNA synthesis in vivo. J. Bacteriol. 102, 204–212 (1970)
Friesen, J.D., Fiil, N.P., Parker, J.M., Haseltine, W.A.: A new relaxed mutant of E. coli with an altered 50S ribosomal subunit. Proc. Natl. Acad. Sci. U.S.A. 71, 3465–3469 (1974)
Gallant, J., Harada, B.: The control of ribonucleic acid synthesis in E. coli III. The functional relationship between purine ribonucleoside triphosphate pool sizes and the rate of ribonucleic acid accumulation. J. Biol. Chem. 244, 3125–3132 (1969)
Gallant, J., Lazzarini, R.: The regulation of ribosomal RNA synthesis and degradation in bacteria. In: Protein synthesis, a series of advances, 2 (E.H. McConkey, ed.), New York: M. Dekker 1976
Gallant, J., Palmer, L., Pao, C.C.: Anomalous synthesis of ppGpp in growing cells. Cell 11, 181–185 (1977)
Ginsburg, D., Steitz, J.A.: The 30S ribosomal precursor RNA from E. coli: A primary transcript containing 23S, 16S and 5S sequences. J. Biol. Chem. 250, 5647–5654 (1975)
Ikemura, T., Dahlberg, J.E.: Small ribonucleic acids of E. coli I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J. Biol. Chem. 248, 5024–5032 (1973)
Ikemura, T., Dahlberg, J.E.: Small ribonucleic acids of E. coli II. Noncoordinate accumulation during stringent control. J. Biol. Chem. 248, 5033–5041 (1973)
Ikemura, T., Nomura, M.: Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid colE1 plasmids in E. coli. Cell 11, 779–793 (1977)
Khan, S.R., Yamazaki, H.: Correlation between guanosine tetraphosphate accumulation and degree of amino acid control of ribonucleic acid accumulation during nutritionally slowed growth in E. coli. Biochemistry 13, 2785–2788 (1974)
Laffler, T., Gallant, J.: SpoT, a new genetic locus involved in the stringent response in E. coli. Cell 1, 27–30 (1974)
Lavalle, R., Desmarez, L., DeHauwer, G.: Natural messenger translation impairment in an E. coli mutant. In: Control of ribosome synthesis (N.C. Kjeldgaard and O. Maaloe, eds.). New York: Academic Press 1976
Lund, E., Dahlberg, J.E., Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell 7, 165–177 (1976)
Lund, E., Dahlberg, J.E.: Spacer transfer RNAs in ribosomal RNA transcripts of E. coli: Processing of 30S ribosomal RNA in vitro. Cell 11, 247–262 (1977)
Mosteller, R., Yanofsky, C.: Transcription of the tryptophan operon in E. coli: Rifampicin as an inhibitor of initiation. J. Mol. Biol. 48, 525–531 (1970)
Neidhardt, F.C.: Culture medium for enterobacteria. J. Bacteriol. 119, 736–747 (1974)
Peacock, A.C., Dingman, C.W.: Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agaroseacrylamide composite gels. Biochem. 7, 668–674 (1968)
Schaechter, M., Maaloe, O., Kjeldgaard, N.C.: Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. J. Gen. Microbiol. 19, 592–606 (1958)
Stamminger, G., Lazzarini, R.A.: Altered metabolism of the guanosine tetraphosphare, ppGpp, in mutants of E. coli. Cell 1, 85–90 (1974)
Yarus, M.: Isolation and properties of a plasmid which expresses the E. coli Su+7 amber suppressor tRNA gene. Mol. Gen. Genet. 170, 291–298 (1979a)
Yarus, M., Eisenberg, S.P., Soll, L.: The structure of the DNA containing the E. coli tRNATry gene. Mol. Gen. Genet. 170, 299–308 (1979b)
Yarus, M., Cline, S.W.: The structure of the ϕ80d3 ilv+ Su+7 transducing phage and the origin of its Su+7 tRNA gene containing fragment. Mol. Gen. Genet. 170, 283–289 (1979c)
Communicated by H.W. Boyer
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Yarus, M. Relaxation of stable RNA synthesis by a plasmid-borne locus. Molec. Gen. Genet. 170, 309–317 (1979). https://doi.org/10.1007/BF00267064
- Ribosomal RNAs
- State Level
- Steady State Level
- tRNA Gene