Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

A site of action for tRNA mediated regulation of the ilvOEDA operon of Escherichia coli K12


Transfer RNA (tRNA), rho factor, threonine deaminase and the ilvO locus are molecular participants in the regulation of isoleucine-valine (ilv) biosynthesis. Isogenic strains have been constructed with the hisT76 mutation in pairwise combination with ilvO mutations, the rho221 mutation and the ilvDAC115 deletion mutation. The role of the altered tRNA of the hisT76 mutation was found to be independent of the sites of action of the ilvO - mutation, rho factor, and threonine deaminase. The expression of the ilvOEDA operon is stimulated 2-fold when the hisT76 mutation is present in strains containing either ilvO - or rho221 mutations. The expression of the ilvOEDA operon remains nonrepressed in a hisT76 strain deleted for threonine deaminase. These results indicate that the hisT76 undermodified tRNAs are influencing the initiation of transcription of the ilvOEDA operon.

This is a preview of subscription content, log in to check access.


  1. Adhya, S., Gottesman, M.: Control of transcription termination. Ann. Rev. Biochem. 47, 967–996 (1978)

  2. Arfin, S.M., Ratzkin, B., Umbarger, H.E.: The metabolism of valine and isoleucine in Escherichia coli XVII the role of induction in the derepression of acetohydroxy acid isomeroreductase. Biochem. biophys. Res. Commun. 37, 902–908 (1969)

  3. Bachmann, B.J., Low, K.B., Taylor, A.N.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)

  4. Bertrand, K., Korn, L., Lee, F., Platt, T., Squires, C.L., Squires, C., Yanofsky, C.: New features of the regulation of the tryptophan operon. Science 189, 22–26 (1975)

  5. Brenchley, J.F., Williams, L.S.: Transfer RNA involvement in the regulation of enzyme synthesis. Ann. Rev. Microbiol. 29, 251–274 (1975)

  6. Brenner, M., Ames, B.N.: Histidine regulation in Salmonella typhimurium. IX. Histidine transfer ribonucleic acid of its regulatory mutants. J. biol. Chem. 247, 1080–1088 (1972)

  7. Bruni, C.B., Colantuoni, V., Sbordone, L., Cortese, R., Blasi, F.: Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants. J. Bact. 130, 4–10 (1977)

  8. Calhoun, D.H., Hatfield, G.W.: Autoregulation: a role for a biosynthetic enzyme in the control of gene expression. Proc. nat. Acad. Sci. (Wash.) 70, 2757–2761 (1973)

  9. Calhoun, D.H., Hatfield, G.W.: Autoregulation of gene expression. Ann. Rev. Microbiol. 29, 275–299 (1975)

  10. Childs, G., Sonnenberg, F., Freundlich, M.: Detection of messenger RNA from the isoleucine-valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: Involvement of transfer RNA in transcriptional repression. Molec. gen. Genet. 151, 121–126 (1977)

  11. Cohen, B.N., Jones, E.W.: New map location of ilvO in Escherichia coli. Genetics 83, 201–225 (1976)

  12. Cortese, R., Landsberg, R., Vonder Haar, R.A., Umbarger, H.E., Ames, B.N.: Pleiotropy of hisT mutants blocked in pseudouridine synthesis in tRNA: leucine and isoleucine-valine operons. Proc. nat. Acad. Sci. (Wash.) 71, 1857–1861 (11974)

  13. Duggan, E.E., Wechsler, J.H.: An assay for transaminase B enzyme activity in Escherichia coli K-12. Analyt. Biochem. 51, 67–79 (1973)

  14. Eidlic, L., Neidhardt, F.C.: Role of valyl-tRNA synthetase in enzyme repression. Proc. nat. Acad. Sci. (Wash.) 53, 539–543 (1965)

  15. Ely, B.: Physiological studies of Salmonella histidine operatorpromoter mutants. Genetics 78, 593–606 (1974)

  16. Favre, R., Wiater, A., Puppo, S., Iaccarino, M.: Expression of a valine-resistant acetolactate synthetase activity mediated by an ilvO and ilvG genes of Escherichia coli K-12. Molec. gen. Genet. 143, 243–252 (1976)

  17. Glover, S.W.: Valine resistant mutants of Escherichia coli K-12. Genet. Res. 3, 448–460 (1962)

  18. Guardiola, J., Cervone, F., Lamberti, A., Levinthal, M., Iaccarino, M.: Dual autogenous regulatory role of threonine deaminase in Escherichia coli K-12. Molec. gen. Genet. 159, 27–32 (1978)

  19. Hatfield, G.W., Burns, R.O.: The specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: Its implication in repression. Proc. nat. Acad. Sci. (Wash.) 66, 1027–1035 (1970)

  20. Iaccarino, M., Berg, P.: Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase. J. Bact. 105, 527–537 (1971)

  21. Kasai, T.: Regulation of the expression of the histidine operon in Salmonella typhimurium. Nature (Lond.) 249, 523–527 (1974)

  22. Korn, L.J., Yanofsky, C.: Polarity suppressors increase expression of the wild type tryptophan operon of Escherichia coli. J. molec. Biol. 103, 395–409 (1976)

  23. Lawther, R.P., Hatfield, G.W.: Biochemical characterization of an Escherichia coli hisT strain. J. Bact. 130, 552–557 (1977)

  24. Levinthal, M., Levinthal, M., Williams, L.S.: The regulation of the ilvADGE operon: Evidence for positive control by threonine deaminase. J. molec. Biol. 102, 453–465 (1976)

  25. Levinthal, M., Williams, L.S., Levinthal, M., Umbarger, H.E.: Role of threonine deaminase in the regulation of isoleucine and valine biosynthesis. Nature (Lond.) New Biol. 246, 65–68 (1973)

  26. Low, B., Gates, F., Goldstein, T., Söll, D.: Isolation and partial characterization of temperature sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases. J. Bact. 108, 742–750 (1971)

  27. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

  28. Miller, J.H.: Experiments in molecular genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1972

  29. Morse, D.E., Morse, A.N.C.: Dual-control of the tryptophan operon is mediated by both tryptophanyl-tRNA synthetase and the repressor. J. molec. Biol. 103, 209–226 (1976)

  30. Munger, J.E.: A comparison of the affinity for L-threonine deaminase between transfer RNA from wild type Salmonella typhimurium LT2 and transfer RNA from hisT Salmonella typhimurium mutants. J. Undergrad. Res. Biol. Sci. UCI 5, 108–125 (1976)

  31. Neidhardt, F.C., Parker, J., McKeever, W.G.: Function and regulation of aminoacyl-tRNA synthetases in prokaryotic and eukaryotic cells. Ann. Rev. Microbiol. 29, 215–250 (1975)

  32. Ramakrishnan, T., Adelberg, E.A.: Regulatory mechanisms in the biosynthesis of isoleucine and valine II. Identification of two operator genes. J. Bact. 89, 654–660 (1965)

  33. Rizzino, A.A., Bresalier, R.S., Freundlich, M.: Derepressed levels of the isoleucine-valine and leucine enzymes in hisT1504, a strain of Salmonella typhimurium with altered leucine transfer ribonucleic acid. J. Bact. 117, 449–455 (1974)

  34. Roberts, J.W.: Transcription termination and its control in E. coli. In: RNA polymerase (Losick, R. and Chamberlin, M., eds.) Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1976

  35. Rosner, J.L.: Formation, induction, and curing of bacteriophage P1 lysogens. Virology 48, 679–689 (1972)

  36. Singer, C.E., Smith, G.R., Cortese, R., Ames, B.N.: Mutant tRNAhis ineffective in repression and lacking two pseudouridine modifications. Nature (Lond.) New Biol. 238, 72–74 (1972)

  37. Smith, J.M., Smolin, D.E., Umbarger, H.E.: Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12. Molec. gen. Genet. 148, 111–124 (1976)

  38. Szentirmai, A., Szentirmai, M., Umbarger, H.E.: Isoleucine and valine metabolism of Escherichia coli XV. Biochemical properties of mutants resistant to thiaisoleucine. J. Bact. 95, 1672–1679 (1968)

  39. Wasmuth, J.J., Umbarger, H.E.: Effect of isoleucine, valine, or leucine starvation on the potential for formation of branched-chain amino acid biosynthetic enzymes. J. Bact. 116, 548–561 (1973)

  40. Yanofsky, C., Söll, L.: Mutations affecting tRNAtrp and its charging and their effect on regulation of transcription termination at the attenuator of the trp operon. J. molec. Biol. 113, 663–677 (1977)

Download references

Author information

Additional information

Communicated by G. O'Donovan

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lawther, R.P., Hatfield, G.W. A site of action for tRNA mediated regulation of the ilvOEDA operon of Escherichia coli K12. Molec. Gen. Genet. 167, 227–234 (1978). https://doi.org/10.1007/BF00266916

Download citation


  • Escherichia Coli
  • Threonine
  • Deletion Mutation
  • hisT76 Strain
  • Pairwise Combination