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Role of an acid phosphatase isoenzyme in callus tissue during cytodifferentiation

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Summary

Both histological and isoenzyme patterns of acid phosphatase were observed in callus tissue of Vigna unquiculate (L.) Walp. up to the tenth passage from its initiation. It was observed that when un-differentiated cells begin to transform into a differentiated condition in the form of tracheids, and xylem vessels, a new acid phosphatase band appears at the anionic end of the polyacrylamide gel. The transformation of living cells into a dead, empty tracheid during cellular differentiation and the biosynthesis of the acid phosphatase enzyme are functionally related to the autolysis of the cell contents and lignin synthesis.

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References

  1. Arnison PG, Boll WG (1978) The effect of 2,4-D and kinetin on the activity and isoenzyme pattern of various enzymes in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris Cv. ‘Contender’). Can J Bot 56:2185–2195

  2. Bornman CH (1974) Cytodifferentiation in tissue culture. In: Street HE (ed) Tissue culture and plant science. Academic Press, London New York, p 43

  3. Brown R, Robinson E (1955) Cellular differentiation and development of enzyme protein in plant protein. In: Butlet EF (ed) Biological specificity and growth. Princeton University Press, Princeton, p 93

  4. Davis BJ (1964) Disc electrophoresis II. Ann NY Acad Sci 121:404–427

  5. Gahan PB, Maple AJ (1966) The behaviour of lysosome-like particles during cell differentiation. J Exp Bot 17:151–155

  6. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin-phenol reagent. J Biol Chem 193:265–275

  7. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479

  8. Scandalios JG (1974) Isoenzyme in development and differentiation. Annu Rev Plant Physiol 25:225–258

  9. Torrey JG, Fosket DE (1970) Cell division in relation to cytodifferentiation in cultured pea root segments. Am J Bot 59:1072–1080

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Communicated by D. von Wettstein

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De, K.K., Roy, S.C. Role of an acid phosphatase isoenzyme in callus tissue during cytodifferentiation. Theoret. Appl. Genetics 68, 285–287 (1984). https://doi.org/10.1007/BF00266904

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Key words

  • Acid phosphatase
  • Callus tissue
  • Differentiation