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Immunohistochemical localization of thymosin β9 in bovine tissues

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Using an indirect fluorescent antibody technique with frozen sections, the localization of thymosin β9 was investigated for the first time in bovine thymus, spleen, lung, muscle and liver. The antibodies used have been raised against the N-terminal fragment 1–14 of thymosin β9 in order to minimize the cross-reactivity with thymosin β4 which was found to be also present in bovine tissues. The specific antibodies against thymosin β9 raised in our laboratory allowed us to localize this peptide in presence of the highly homologous and always accompanying thymosin β4 in different tissues. Although thymosin β9 was first isolated from calf thymus, it could be also detected in other bovine organs. The highest density of positive immunoreaction was found to be in spleen sections. In the muscle tissue a pronounced fluorescence intensity was present in the region of the sarcolemn.

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  1. Erickson-Viitanen S, Ruggieri S, Natalini P, Horecker BL (1983) Distribution of thymosin β4 in vertebrate classes. Arch Biochem Biophys 221:570–576

  2. Hannappel E, Davoust S, Horecker BL (1982) Thymosin β8 and β9; two new peptides isolated from calf thymus homologous to thymosin β4. Proc Natl Acad Sci USA 79:1708–1711

  3. Hannappel E (1986) One-step procedure for the determination of thymosin β4 in small tissue samples and its separation from other thymosin β4-like peptides by high-pressure liquid chromatography. Anal Biochem 156:390–396

  4. Hannappel E (1987) Thymosin β4- und β4-artige Peptide. In: Hagen ED (ed) Thymusfaktoren, Thymuspräparate. Biologische Eigenschaften und klinische Aspekte. Gustav Fischer Verlag, Stuttgart New York, pp 64–73

  5. Low TLK, Hu S-K, Goldstein AL (1981) Complete amino acid sequence of thymosin β4. A thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations. Proc Natl Acad Sci USA 78:1162–1166

  6. Mihelić M, Kalbacher H, Hannappel E, Voelter W (1989) MicroELISA method for the determination of thymosin β9 discriminating between thymosin β9 and the structurally closely related thymosin β4. J Immunol Methods 122:7–13

  7. Rebar RW, Miyake A, Low TLK, Goldstein AL (1981) Thymosin stimulates secretion of luteinizing hormone-releasing factor. Science 214:669–671

  8. Thurman GB, Low TLK, Rossio J, Goldstein AL (1981) Specific and nonspecific macrophage migration inhibition. In: Goldstein AL, Chrigos MA (eds) Lymphokines and thymic hormones: their potential utilization in cancer therapeutics. Raven Press, New York, pp 145–157

  9. Voelter W, Kalbacher H, Mihelić M, Hannappel E (1986) Peptides of the thymus; isolation, synthesis and their activity in the rosette assay. In: Wünsch E, Moroder L (eds) Proceedings of the München-Shanghai-Symposium on Peptide and Protein Chemistry, p 114

  10. Wei NR, Giebel W (1988) Spezifische und unspecifische Fluoreszenz bei Verwendung von Gefrierschnitten der Cochlea. Laryngol Rhinol Otol (Stuttg) 67:513–517

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Correspondence to W. Voelter.

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Mihelić, M., Giebel, W., Wei, N.-. et al. Immunohistochemical localization of thymosin β9 in bovine tissues. Histochemistry 95, 175–178 (1990). https://doi.org/10.1007/BF00266590

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  • Public Health
  • Peptide
  • Fluorescence Intensity
  • Muscle Tissue
  • Specific Antibody