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Production of lipid from starch by a nitrogen-controlled mixed culture of Saccharomycopsis fibuliger and Rhodosporidium toruloides

Summary

Production of lipid from starch in a two-component mixed culture in a fed batch process was studied. Saccharomycopsis fibuliger was used as the amylolytic microorganism and Rhodosporidium toruloides, which does not grow on starch, as the lipid producer. The concentration of nitrogen can be used as an external regulator of growth and competition in this process. The total biomass concentration decreased and the relative amount of Rh. toruloides increased with decreasing initial concentration of nitrogen in the medium. The highest lipid concentration (9.7 g·l-1), highest overall lipid production rate (0.15 g·l-1·h-1) and highest concentration of lipid in biomass (36.5%) were obtained in cultures with an initial nitrogen concentration of 0.5 g·l-1. Compared to monocultures of Lipomyces starkeyi and Aspergillus oryzae on starch, the mixed culture showed slightly lower conversion of starch to lipid but was superior in the final lipid concentration and the overall lipid production rate.

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References

  1. Azoulay E, Janssens J, Raphael A, Bertrand JC (1980) Growth of C. tropicalis on starch and cassava powder for the obtention of high-protein feed. 6th International Fermentation Symposium, London, Ontario, Canada

  2. Chaney AL, Marbach EP (1962) Modified reagent for determination of urea and ammonia. Clin Chem 8:130–132

  3. Choi SY, Ryu DY, Rhee JS (1982) Production of microbial lipids: Effects of growth rate and oxygen on lipid synthesis and fatty acid composition of Rhodotorula gracilis. Biotechnol Bioeng 24:1165–1172

  4. Dostálek M, Häggström MH (1983) Mixed culture of Saccharomycopsis fibuliger and Zymomonas mobilis on starch —Use of oxygen as regulator. Eur J Microbiol Biotechnol 17:269–274

  5. Guerzoni ME, Lambertini P, Lercker G, Marchetti R (1985) Technological potential of some starch-degrading yeasts. Starch/Stärke 37:52–57

  6. Häggström MH, Dostálek M (1981) Regulation of mixed culture of Streptococcus lactis and Saccharomycopsis fibuliger. Eur J Microbiol Biotechnol 12:216–219

  7. Jarl K (1969) Symba yeast process. Food Technol 23:1009–1012

  8. Kaur P, Worgan JT (1982) Lipid production by Aspergillus oryzae from starch substrates. Eur J Microbiol Biotechnol 16:126–130

  9. Krumphanzl V, Grégr V, Pelechová J, Uher J (1973) Biomass and fat production in Rhodotorula gracilis. Adv Microb Engineering, Part 1. Biotechnol Bioeng Symposia No 4:245–256

  10. Lemmel SA, Heimsch RC, Edwards LL (1979) Optimizing the continuous production of Candida utilis and Saccharomycopsis fibuliger on potato processing waste water. Appl Environ Microbiol 37:227–232

  11. Miller GL, Blum R, Glennon WE, Burton AL (1960) Measurement of carboxymethylcellulase activity. Anal Biochem 2:127–132

  12. Misra S, Amitabha G, Dutta J (1984) Production and composition of microbial fat from Rhodotorula glutinis. J Sci Food Agric 35:59–65

  13. Ratledge C (1978) Primary products of metabolism — Lipids and fatty acids. Economic Microbiology 2:263–302

  14. Ratledge C (1982) Microbial oils and fats: an assessement of their commercial potential. Progress Ind Microb 16:119–206

  15. Wickerham LJ, Lookwood L B, Pettijohn O G, Ward G E (1944) Starch hydrolysis and fermentation by yeast Endomycopsis fibuliger. J Bacteriol 48:413–442

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Correspondence to Milan Dostálek.

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Dostálek, M. Production of lipid from starch by a nitrogen-controlled mixed culture of Saccharomycopsis fibuliger and Rhodosporidium toruloides . Appl Microbiol Biotechnol 24, 19–23 (1986). https://doi.org/10.1007/BF00266279

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Keywords

  • Lipid
  • Biomass
  • Starch
  • Aspergillus
  • Mixed Culture