Genetic control of the major zein polypeptides in maize (Zea mays L.) was studied by isoelectric focusing (IEF) in agarose. Linkage relationships were determined by making a number of crosses, then determining the expression of zein polypeptides in backcross seeds. Chromosome linkages were determined by using the markers sugary-1 (for chromosome 4), yellow-8, and a waxy 7–9 translocation (for chromosome 7). Nine zeins were in one linkage group on chromosome 4, six in another linkage group on chromosome 4, and four zeins were in one linkage group on chromosome 7. Some IEF single bands consisted of at least two polypeptides, which were detected by subsequent sodium dodecyl sulfate polyacrylamide gel electrophoresis, by aberrant ratios in backcrosses, or by differing recombination percentages. One zein occurred only in homozygous sugary-1 seeds. Three sets of closely-linked zeins were noted that occurred together almost exclusively in certain inbreds.
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Cooperative investigations of the U.S. Department of Agriculture, Agricultural Research Service, and the Illinois Agricultural Experiment Station, Department of Agronomy, University of Illinois, Urbana, USA
Mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned
Communicated by A. L. Kahler
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Wilson, C.M., Sprague, G.F. & Nelsen, T.C. Linkages among zein genes determined by isoelectric focusing. Theoret. Appl. Genetics 77, 217–226 (1989). https://doi.org/10.1007/BF00266190
- Zea mays L.
- Genetic linkages
- Isoelectric focusing