Out of seven chlorinated aliphatic hydrocarbons tested, only trans-1,2-dichloroethene was relatively non-toxic for a mixed methanotrophic culture. The compound was degraded at a rate of 0.4 μmol/mg protein·h-1 and liberation of inorganic chloride was observed. Trans-2,3-dichlorooxirane was formed as an intermediate which was converted further only by chemical transformation with a half life of 31 h. From the consortium, a pure culture was isolated and found to be capable of degradation of trans-1,2-dichloroethene when grown in the presence of methane or methanol. The ability of cometabolic degradation of this compound was not specific for this isolate, since Methylomonas methanica NCIB11130 and Methylosinus trichosporium OB3b also showed degradation of trans-1,2-dichloroethene when grown with methane as sole carbon source.
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proton nuclear magnetic resonance
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Janssen, D.B., Grobben, G., Hoekstra, R. et al. Degradation of trans-1,2-dichloroethene by mixed and pure cultures of methanotrophic bacteria. Appl Microbiol Biotechnol 29, 392–399 (1988). https://doi.org/10.1007/BF00265825
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