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Isolation and characterization of anthocyanin variants originating from the unstable system an2-1 in Petunia hybrida (Hort.)

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Forty stable an2-n alleles, derived from the unstable system an2-1, have been tested for anthocyanin synthesis. All of them proved to be different from both the An2 and an2 natural alleles. Only two were distinct from the others which according to Duncan's multiple range test formed a group of overlapping populations. Amongst the variants isolated there was a large majority of light-coloured types. Regulation-like effects of the an2-n alleles on the subsequent genes of the anthocyanin pathway have been observed. A hypothesis concerning the nature of the genetic events occurring at the An2 locus is discussed.

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  1. Bianchi, F.; Cornelissen, P.T.J.; Gerats, A.G.M.; Hogervorst, J.M.W.: Regulation of gene action in Petunia hybrida: unstable alleles of a gene for flower colour. Theor. Appl. Genet. 53, 157–167 (1978)

  2. Britten, R.J.; Davidson, E.H.: Gene regulation for higher cells: a theory. Science 165, 349–357 (1969)

  3. Cornu, A.: Systèmes instables induits chez le Petunia: Mut. Res. 42, 235–248(1977)

  4. Dodds, K.S.; Long, D.H.: The inheritance of colour in diploid potatoes. II. A three factor linkage group. J. Genet. 54, 27–41 (1956)

  5. Dooner, H.K.; Nelson, O.E.: Genetic control of UDP glucose: Flavonol 3-0-glucosyl transferase in the endosperm of maize. Biochem. Genet. 15, 509–519 (1977)

  6. Emerson, R.A.: The frequency of somatic mutation in variegated pericarp in maize. Genetics 14, 488–511 (1929)

  7. Fincham, J.R.S.; Harrison, B.J.: Instability at the pal locus in Antirrhinum majus. II. Multiple alleles produced by mutation of the original unstable allele. Heredity 22, 211–224 (1967)

  8. Kho, K.F.F.; Bolsman-Louwen, A.C.; Vuik, J.C.; Bennink, G.J.K.: Anthocyanin synthesis in a white flowering mutant of Petunia hybrida. II. Accumulation of dihydroflavonol intermediates in white flowering mutants: Uptake of intermediates in isolated corollas and conversion into anthocyanins. Planta 135, 109–118 (1977)

  9. Kho, K.F.F.; Kamsteeg, J.; Van Brederode, J.: Identification, properties and genetic control of UDP-glucose: cyanidin 3-0-glucosyl transferase in Petunia hybrida. Z. Pflanzenphysiol. 88, 449–464(1978)

  10. McClintock, B.: The control of gene action in maize. Brookhaven Symp. Biol. 18, 162–184 (1965)

  11. Nevers, P.; Saedler, H.: Transposable genetic elements as agents of gene instability and chromosomal rearrangements. Nature 268, 109–115(1977)

  12. Nybom, N.: Thin layer chromatographic analysis of anthocyanidins. Physiol. Plant. 17, 217–225 (1964)

  13. Peterson, P.A.: The En mutable system in maize. III. Transposition associated with mutational events. Theor. Appl. Genet. 40, 367–377 (1970)

  14. Reddy, A.R.; Peterson, P.A.: Germinal derivatives of the En controlling element system in maize: characterization of colored, pale and colorless derivatives of a2-m. Theor. Appl. Genet. 48, 269–278 (1976)

  15. Stadler, L.J.: Spontaneous mutation at the R locus in maize. II. Race difference in mutation rate. Amer. Nat. 82, 289–314 (1948)

  16. Stadler, L.J.: Spontaneous mutation in maize. Cold Spring Habor Symposium Quant. Biol. 16, 49–63 (1951)

  17. Tabak, A.C.J.; Meyer, H.; Bennink, G.J.H.: Modification of the B ring during flavonoid synthesis in Petunia hybrida: Introduction of the 3′-hydroxyl group regulated by the gene Ht1, Planta, 139, 67–71 (1978)

  18. Wiering, H.: Genetics of flower colour in Petunia hybrida Hort. Genen en Phaenen 17, 117–134 (1974)

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Communicated by H.F. Linskens

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Farcy, E., Cornu, A. Isolation and characterization of anthocyanin variants originating from the unstable system an2-1 in Petunia hybrida (Hort.). Theoret. Appl. Genetics 55, 273–278 (1979). https://doi.org/10.1007/BF00265365

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Key words

  • Petunia hybrida
  • Genetic instability
  • Anthocyanin synthesis
  • Allelic series