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Nucleus substitution between Petunia species using gamma ray-induced androgenesis

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The ovaries of two different Petunia species: Petunia hybrida (hort) and Petunia parodii (Steere) were irradiated with τ-ray doses ranging from 50 to 1,000 Gy before pollination. Seed setting occurred after 4 days preculture on a non-sterile medium. Ovaries transformed into fruits were then cultivated aseptically with the following results: (1) τ-ray doses ranging from 200 to 1,000 Gy led to the development of two types of plants: haploids 2n=x=7 and overdiploids 2n>2x=14. (2) The androgenetic origin of haploids was ascertained by using genetic markers. The origin of overdiploids is discussed. (3) Androgenetic haploids contained the chloroplasts of the irradiated female parent. No visible change of cp DNA patterns was observed after irradiation. (4) The four possible androgenetic events were successfully obtained between the two Petunia species: hybrida haploids with hybrida or parodii cytoplasm, and parodii haploids with parodii or hybrida cytoplasm.

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  1. Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403

  2. Boeshore M, Lifshitz I, Hanson MR, Izhar S (1983) Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants. Mol Gen Genet 190:459–467

  3. Cornu A, Maizonnier D (1982) The genetics of Petunia. Plant Breed Rev 1:11–58

  4. De Verna JW, Collins GB (1984) Maternal haploids of Petunia axillaris (Lam) BSP over culture of placenta attached ovules. Theor Appl Genet 69:187–192

  5. Ehrensberger R (1948) Versuche zur Auslösung von Haploidie bei Blütenpflanzen. Biol Zentralbl 67:537–546

  6. Framelaer Y, Cammaerts D, Karp A, Sidorov V, De Brouwer D, Negrutiu I, Jacobs M (1986) Cellular engineering by gamma fusion and egg transformation in nuclear techniques and in vitro culture for plant improvement. IAEA SM 282/44 Vienna: 453–461

  7. Gerassimova H (1936) Experimentell erhaltene haploide Pflanze von Crepis tectorum. Planta 25:696–702

  8. Gleba YY, Hinnisdaels S, Sidorov VR, Kaleda VA, Parokonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna by “gamma fusion”. Theor Appl Genet 76:760–766

  9. Goodsell SR (1961) Male sterility in corn by androgenesis. Crop Sci 227–228

  10. Imamura J, Saul MW, Potrykus J (1987) X-ray irradiation promoted asymmetric somatic hybridization and molecular analysis of products. Theor Appl Genet 74:445–450

  11. Kumar A, Cocking EC, Bovenberg WA, Kool AJ (1982) Restriction endonuclease analysis of chloroplast DNA in interspecies somatic hybrids of Petunia. Theor Appl Genet 62:377–384

  12. Pelletier G, Ferault M, Goujaud J, Vedel F, Caboche M (1987) The use of rootless mutants for the screening of spontaneous androgenetic and gynogenetic haploids in Nicotiana tabacum: evidence for the direct transfer of cytoplasm. Theor Appl Genet 75:13–15

  13. Raquin C (1982) Analyse des ségrégations de gènes impliquées dans la pigmentation florale des Pétunias issus d'androgenèse. CR Acad Sci Paris 274:335–338

  14. Raquin C (1985) Induction of haploid plants by in vitro culture of Petunia ovaries pollinated with irradiated pollen. Z Pflanzenzücht 94:166–169

  15. Raquin C (1986) Induction of haploid and “haploid plus” petunias by combining gametophyte irradiation and in vitro ovary culture. In: Somers DA, Gegenbach BG, Biesboer DD, Hackett WP, Greer CE (eds) 6th Int Congr Plant Tiss cell Culture, Abstr Minneapolis, p 123

  16. Sauton A, Dumas de Vaulx R (1987) Obtention de plantes haploîdes chez le melon (Cucumis melo L) par gynogenèse induite par du pollen irradié. Agronomie 7:141–148

  17. Singh IS, Cornu A (1976) Recherche de plantes haploîdes de Pétunia à noyau androgénétique et à cytoplasme gynogénétique déterminant la stérilité pollinique. Ann Amel Plant 26:565–568

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Communicated by Yu. Gleba

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Raquin, C., Cornu, A., Farcy, E. et al. Nucleus substitution between Petunia species using gamma ray-induced androgenesis. Theoret. Appl. Genetics 78, 337–341 (1989). https://doi.org/10.1007/BF00265293

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Key words

  • Petunia hybrida
  • Petunia parodii
  • Androgenesis
  • Ovary irradiation
  • Chloroplast DNA