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Chromosomal variability in tissue cultures and regenerated plants of Hordeum

Summary

Spontaneous polyploidy, aneuploidy, and chromosomal rearrangements were observed in callus and suspension cultures of Hordeum vulgare, H. jubatum, and their interspecific hybrid. The extent to which each class of chromosomal variability was present in a culture depended upon differentiated state, age, and history. Cytological and isozymic analysis of subdivided callus cultures revealed spatial segregation of chromosomal variability. Cytogenetic analyses were performed to determine the expression of this in vitro chromosomal variability in corresponding regenerated plant tissues. A complete loss of polyploidy and a decrease in aneuploidy and chromosomal rearrangements were observed. Analyses of specific isozyme activities in regenerates suggested that a quantitative segregation of H. vulgare and H. jubatum genomes had occurred in tissue cultures of their interspecific hybrid. Possible uses of in vitro chromosomal variability for plant breeding and genetical studies are discussed.

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Literature

  1. Allard, R.W.; Kahler, A.L.; Weir, B.S.: Isozyme polymorphisms in barley populations. In: Bailey Genetics II (ed. Nilan, R.A.), pp. 1–13. Pullman, Wash.: Washington State University 1970

  2. Asami, H.; Inomata, N.; Okamoto, M.: Chromosome variation in callus cells derived from Secale cereale L. with and without B-chromosome. Jap. J. Genet. 51, 297–303 (1976)

  3. Babbel, G.R.; Wain, R.P.: Genetic structure of Hordeum jubatum L. I. Outcrossing rates and heterozygosity levels. Can. J. Genet. Cytol. 19, 143–152 (1977)

  4. Bassiri, A.: Barley cultivar identification by use of isozyme electrophoretic patterns. Can. J. Plant Sci. 56, 1–6 (1976)

  5. Bayliss, M.W.: The effects of growth in vitro on the chromosome complement of Daucus carota L. suspension cultures. Chromosoma 51, 401–411 (1975)

  6. Cheng, T.Y. Smith, H.H.: Organogenesis from callus culture of Hordeum vulgare. Planta 123, 307–310 (1975)

  7. Cummings, D.P.; Green, C.E.; Stuthman, D.D.: Callus induction and plant regeneration in oats. Crop Sci. 16, 465–470 (1975)

  8. D'Amato, F.: The problem of genetic stability in plant tissue and cell cultures. In: Crop resources for today and tomorrow (eds. Frankel, O.; Hawkes, J.G.), pp. 333–348. Cambridge, U.K.: University Press 1975

  9. Demoise, L.F.; Partanen, C.R.: Effects of subculturing and physical condition of medium on the nuclear behavior of a plant tissue culture. Amer. J. Bot. 56, 147–152 (1969)

  10. Fedak, G.: Allozymes as aids to Canadian barley cultivar identification. Euphytica 23, 166–173 (1975)

  11. Fobes, J.F.: Trisomic analysis of isozymic loci in tomato species: Segregation and dosage effects. Ph.D. Thesis, University of California, Davis, CA (1977)

  12. Gamborg, O.L.; Miller, R.A.; Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 148–151 (1968)

  13. Green, C.E.; Phillips, R.L.: Plant regeneration from tissue cultures of maize. Crop Sci. 15, 417–421 (1975)

  14. Kallak, H.; Jarvekulg, L.: Changes in chromosome complement in long-term pea callus cultures. Acta Biol. Acad. Sci. Hung. 28, 183–189 (1977)

  15. Kao, K.N.: A nuclear staining method for plant protoplasts. In: Plant tissue culture methods, (eds. Gamborg, O.L.; Wetter, L.R.), pp. 60–61. Saskatoon, Sask., Canada: N.R.C.C. 1975

  16. Kao, K.N.; Miller, R.A.; Gamborg, O.L.; Harvey, B.L.: Variations in chromosome number and structure in plant cells grown in suspension cultures. Can. J. Genet. Cytol. 12, 297–301 (1970)

  17. Kasha, K.J.: Haploids from somatic cells. In: Haploids in higher plants: advances and potential (ed. Kasha, K.J.), pp. 67–90. Guelph, Ont., Canada: University of Guelph 1974

  18. Ladizinsky, G.; Fainstein, R.: A case of genome partition in polyploid oats. Theor. Appl. Genet. 51, 159–160 (1978)

  19. Muir, W.A.: Influence of variation in chromosome number and differentiation in plant tissue cultures. In: Proceedings of the International Congress of Plant Tissue Culture (eds. White, P.R.; Grove, A.R.), pp. 485–492. Berkeley, Calif.: McCutchan 1965

  20. Murashige, T.; Nakano, R.: Morphogenetic behavior of tobacco tissue cultures and implication of plant senecence. Amer. J. Bot. 52, 819–827 (1965)

  21. Murry, L.E.: A cytogenetic investigation of X Agrohordeum pilosilemma. Ph.D. Thesis, Michigan State University, E. Lansing, Mich. 1975

  22. Nakai, Y.: Variations of esterase isozymes and some soluble proteins in diploids and their induced autotetraploids in plants. Jap. J. Genet. 52, 171–181 (1977)

  23. Nishi, T.; Mitsuoka, S.: Occurrence of various ploidy plants from anther and ovary culture of rice plant. Jap. J. Genet. 44, 341–346 (1969)

  24. Novak, F.J.; Vystot, B.: Karyology of callus cultures derived from Nicotiana tabacum L. haploids and ploidy of regenerants. Z. Pflanzenzücht. 75, 62–70 (1975)

  25. Ogura, H.: The effects of a morphactin, chloroflurenol, on organ redifferentiation from tobacco calluses cultures in vitro. Bot. Mag. (Tokyo) 88, 1–8 (1975)

  26. Ogura, H.: The cytological chimeras in original regenerates from tobacco tissue cultures and in their offsprings. Jap. J. Genet. 51, 161–174 (1976)

  27. Ogura, H.: Genetic control of chromosomal chimerism found in a regenerate from tobacco callus. Jap. J. Genet. 53, 77–90 (1978)

  28. Orton, T.J.: A quantitative analysis of growth and regeneration from tissue cultures of Hordeum vulgare, H. jubatum, and their interspecific hybrid. Env. Exper. Bot. (in press, 1979)

  29. Partanen, C.R.: Cytological behavior of plant tissues in vitro as a reflection of potentialities in vivo. In: Proceedings of the International Congress of Plant Tissue Culture, (eds. White, P.R.; Grove, A.R.), p. 463–471. Calif.: McCutchan, Berkeley, 1965

  30. Rajathy, T.; Morrison, J.W.: Cytogenetic studies in the genus Hordeum IV. Hybrids of H. jubatum, H. brachyantherum, H. vulgare, and a hexaploid Hordeum sp. Can. J. Genet. Cytol. 1, 124–132 (1959)

  31. Reinert, J.; Backs, D.: Control of totipotency in plant cells growing in vitro. Nature 220, 1340–1341 (1968)

  32. Saalback, B.; Koblitz, H.: Karyological instabilities in callus cultures from haploid barley plants. Biochem. Physiol. Pfl. 171, 469–473 (1977)

  33. Sacristan, M.D.; Melchers, G.: The karyological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Mol. Gen. Genet. 105, 317–333 (1969)

  34. Shimada, T.: Chromosome constitution of tobacco and wheat callus cells. Jap. J. Genet. 46, 235–241 (1971)

  35. Shimada, T.; Sasakuma, T.; Tsunewaki, K.: In vitro culture of wheat tissues. I. Callus formation, organ redifferentiation and single cell culture. Can. J. Genet. Cytol. 11, 294–304 (1969)

  36. Shimada, T.; Tabata, M.: Chromosome numbers in cultured pith tissue of tobacco. Jap. J. Genet 42, 195–201 (1967)

  37. Singh, B.D.; Harvey, B.L.; Kao, K.N.; Miller, R.A.: Karyotypic changes and selection pressure in Haplopappus gracilis suspension cultures. Can. J. Genet. Cytol. 17, 109–116 (1975)

  38. Singh, B.D.; Harvey, B.L.: Cytogenetic studies on Haplopappus gracilis cells cultured on agar and in liquid media. Cytologia 40, 347–354 (1975)

  39. Smith, S.M.; Street, H.E.: The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann. Bot 38, 223–241 (1974)

  40. Sunderland, N.: Nuclear cytology. In: Plant cell and tissue culture, Vol. 2. (ed. Street, H.E.), pp. 177–206. Berkeley, Calif.: University of California Press 1977

  41. Tai, W.: Multipolar meiosis in diploid crested wheatgrass, Agropyron cristatum. Amer. J. Bot. 57, 1160–1169 (1970)

  42. Tang, K.S.; Hart, G.E.: Use of isozyme markers in wheat-rye addition lines and in triticale. Genet. Res. 26, 187–201 (1975)

  43. Torrey, J.G.: Morphogenesis in relation to chromosomal constitution in long-term plant tissue cultures. Physiol. Plant. 20, 265–275 (1967)

  44. Wetter, L.R.; Kao, K.N.: The use of isozymes in distinguishing the sexual and somatic hybrids in callus cultures derived from Nicotiana. Z. Pflanzenphysiol. 80, 455–462 (1976)

  45. Wilson, G.B.; Cheng, K.C.: Segregation and reduction in somatic tissues II. The separation of homologous chromosomes in Trillium species. J. Hered. 40, 3–6 (1949)

  46. Yamane, Y.: Induced differentiation of buckwheat plants from subcultured calluses in vitro. Jap. J. Genet. 49, 139–146 (1974)

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Work supported by a grant to Project 3195 from the Michigan Agricultural Experiment Station and by Grant E4-76-S-02-7528. A004 to P.S. Carlson from the US Department of Energy

Communicated by R.Reley

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Orton, T.J. Chromosomal variability in tissue cultures and regenerated plants of Hordeum . Theoret. Appl. Genetics 56, 101–112 (1980). https://doi.org/10.1007/BF00265080

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Key words

  • Plant tissue culture
  • Cytogenetics
  • Plant breeding
  • Barley