In chickpea, out of three colchicine concentrations and two treatment durations used (combinations of 0.25, 0.05, 0.025% colchicine and 4 and 6 h duration), seed treatment with 0.25% for 4 h proved to be the most effective in producing autotetraploids. Colchicine treatment on seedlings failed. The induced tetraploidy was accompanied by larger leaves, flowers, stomata, pollen grains and seeds. Mean percentage stainable pollen and podset were reduced, but some plants had relatively normal meiosis and produced as many pods as the diploid parent, indicating the potential of induced autotetraploids in chickpea improvement.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bhattacharya SK (1956) Study of autotetraploid Cajanus cajan (Linn.) Millsp. Caryologia 9:149–161
Hagberg A, Ellerstrom S (1959) The competition between diploid, tetraploid and aneuploid rye, theoretical and practical aspects. Hereditas 45:369–416
Hilpert G (1957) Effect of selection for meiotic behaviour in autotetraploid rye. Hereditas 43:318–322
Iyengar NK (1939) Cytological investigations on the genus Cicer. Ann Bot (London) 3:271–306
Kumar LSS (1945) A comparative study of autotetraploid and diploid types of green gram (Phaseolus radiatus L.). Proc Indian Acad Sci 21:266–268
Kumar Sen N, Chedda HR (1958) Colchicine induced tetraploids of five varieties of black gram. Indian J Genet 18:238–248
Ladizinsky G, Adler A (1976) Genetic relationships among the annual species of Cicer L. Theor Appl Genet 48:197–203
Phadnis BA, Narkhede MN (1972) Chromosome behaviour in colchicine induced autotetraploids of Cicer arietinum L. Cytologia 37:415–421
Ramanujam S, Joshi AB (1941) Colchicine induced polyploidy in crop plants. 1. Gram (Cicer arietioum L.). Indian J Agric Sci 9:835–849
Sohoo MS, Athwal DS, Chandra S (1970) Colchicine induced polyploidy in chickpeas (Cicer arietinum L.). Theor Appl Genet 40:163–168
Stebbins GL (1950) Variation and evolution in plants. Columbia University Press, Columbia
Thompson MM (1962) Cytogenetics of Rubus 3. Meiotic instability in some higher polyploids. Am J Bot 49:575–587
van der Maesen LJG, Pundir RPS, Remanandan P (1980) The current status of chickpea germplasm work at ICRISAT. In: Proc Int Workshop on Chickpea Improvement. ICRISAT, Patancheru, India, pp 28–32
Approved as J. A. No. 265 by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
Communicated by G. S. Khush
About this article
Cite this article
Pundir, R.P.S., Rao, N.K. & van der Maesen, L.J.G. Induced autotetraploidy in chickpea (Cicer arietinum L.). Theoret. Appl. Genetics 65, 119–122 (1983). https://doi.org/10.1007/BF00264878