Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Studies on plasmid replication

IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid

Summary

This paper describes a complementation test system for replication-defective S. aureus penicillinase plasmids in which the incompatibility barrier has been overcome by the isolation of an incompatibility-defective (Inc) plasmid. This plasmid appears to be stably and irreversibly integrated into the host chromosome as attempts to restore it to its original independent state have been unsuccessful. The Inc plasmid was able to complement the thermosensitive replication defects of Seg plasmids belonging to the same original incompatibility class but was unable to complement ones belonging to a different incompatibility class. Positive and negative phenotypic complementation tests were confirmed at the molecular level by isotopic labeling of plasmid-specific DNA molecules.

This is a preview of subscription content, log in to check access.

References

  1. Achtman, M.: Genetics of the F sex factor. In: Current topics in microbiology and immunology by Arber, W., et al., p. 79–123. Berlin-Heidelberg-New York: Springer 1973

  2. Clark, A. J.: Genetic analysis of a “double male” strain of Escherichia coli. Genetics 48, 105–120 (1963)

  3. Cuzin, F., Jacob, F.: Analyse génétique fonctionelle de l'épisome sexuel d'Escherichia coli K12. C. R. Acad. Sci. (Paris) 260, 2087–2090 (1965)

  4. DeVries, J. K., Maas, W. K.: Description of an incompatibility mutant of Escherichia coli. J. Bact. 115, 213–200 (1973)

  5. Hirota, Y., Ryter, A., Jacob, F.: Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spr. Harb. Symp. quant. Biol. 33, 677–693 (1968)

  6. Jacob, F., Brenner, S., Cuzin, F.: On the regulation of DNA replication in bacteria. Cold Spr. Harb. Symp. quant. Biol. 28, 329–348 (1963)

  7. Johnston, L. H., Dyke, K. G. H.: Stability of penicillinase plasmids in Staphylococcus aureus. J. Bact. 107, 68–73 (1971)

  8. Kingsbury, D. T., Helinski, D. R.: Temperature sensitive mutants for the replication of plasmids in Escherichia coli. I. Isolation and specificity of host and plasmid mutations. Genetics 74, 1–16 (1973)

  9. Maas, R.: Exclusion of an F'lac episome by an Hfr gene. Proc. nat. Acad. Sci. (Wash.) 50, 1051–1055 (1963)

  10. Maas, W. K., Goldschmidt, A. D.: A mutant of Escherichia coli permitting replication of two F factors. Proc. nat. Acad. Sci. (Wash.) 62, 873–880 (1969)

  11. Morrison, T. G., Malamy, M. H.: Comparisons of F factors and R factors: Existence of independent regulation groups in F factors. J. Bact. 103, 81–88 (1970)

  12. Novick, R. P.: Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. J. gen. Microbiol. 33, 121–136 (1963)

  13. Novick, R. P.: Mutations affecting replication and maintenance of penicillinase plasmids in Staphylococcus aureus. Proc. Fifth Int. Congr. Chemother., p. 269–273. Vienna: Verlag der Wiener Medizinischen Akademie (1967a)

  14. Novick, R. P.: Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33, 155–166 (1967b)

  15. Novick, R. P.: Penicillinase plasmids of Staphylococcus aureus. Fed. Proc. 26, 29–38 (1967c)

  16. Novick, R. P.: Extrachromosomal inheritance in bacterial. Bact. Rev. 33, 210–263 (1969)

  17. Novick, R. P.: Studies on Plasmid Replication. III. Isolation and characterization of replication-defective mutants. Molec. gen. Genet. 135, 131–147 (1974)

  18. Novick, R. P., Bouanchaud, D.: Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann. N. Y. Acad. Sci. 182, 279–294 (1971)

  19. Novick, R. P., Brodsky, R.: Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus. J. molec. Biol. 68, 285–302 (1972)

  20. Novick, R. P., Richmond, M. H.: Nature and interactions of the genetic elements governing penicillinase synthesis in S. aureus. J. Bact. 90, 467–480 (1965)

  21. Ohtsubo, E., Nishimura, Y., Hirota, Y.: Transfer defective mutants of sex factors in E. coli. I. Defective mutants and complementation analysis. Genetics 64, 173–188 (1970)

  22. Peyru, G., Wexler, L., Novick, R. P.: Naturally-occurring penicillinase plasmids in Staphylococcus aureus. J. Bact. 98, 215–221 (1969)

  23. Richmond, M. H.: A second regulatory region involved in penicillinase synthesis in Staphylococcus aureus. J. molec. Biol. 26, 357–360 (1967)

  24. Signer, E. R.: Lysogeny: the integration problem. Ann. Rev. Microbiol. 22, 451–488 (1968)

  25. Smith, K., Novick, R. P.: Genetic studies on plasmid-linked cadmium resistance in Staphylococcus aureus. J. Bact. 112, 761–772 (1972)

  26. Wyman, L., Goering, R. V., Novick, R. P.: Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics 76, 681–702 (1974)

Download references

Author information

Additional information

The results presented here were included by L. Wyman in her Ph.D. dissertation in the Dept. of Microbiology, New York University School of Medicine, June, 1971.

Communicated by H. Ozeki

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wyman, L., Novick, R.P. Studies on plasmid replication. Molec. Gen. Genet. 135, 149–161 (1974). https://doi.org/10.1007/BF00264782

Download citation

Keywords

  • Molecular Level
  • Independent State
  • Isotopic Label
  • Complementation Test
  • Plasmid Replication