Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The influence and possible recombination of genotypes on the production of microspore embryoids in anther cultures of Solanum tuberosum and dihaploid hybrids


In addition to physical and chemical factors, genotype appears to be a very important factor influencing success in anther culture. Recombination by making crosses with selected responding clones has been introduced as a possible helpful method to positively influence the success and response type via the factor genotype. From the progeny of such a cross, one genotype could be selected, producing in 30 to 40 percent of the cultured anthers, fully developed embryoids and plantlets, which are a mixture of polyploids, dihaploids and monohaploids.

Further, a pleiotropic marker ‘embryo spot’ visible as a ‘nodal band’ in the plant stage, has been used to confirm the microsporic origin of dihaploids and polyploids and to prove their homozygous nature. This marker also shows potential use in confirming the origin of calli from individual microspores.

This is a preview of subscription content, log in to check access.


  1. van Breukelen, E.W.M.; Ramanna, M.S.; Heimsen, J.G.Th.: Pathenogenetic monohaploids (2n=x=12) from Solanum tuberosum L. and S. verrucosum Schlechtd. and the production of homozygous potato diploids. Euphytica 26, 263–272 (1977)

  2. Corduan, G.: Regeneration of anther derived plants of Hyoscyamus niger L. Planta 127, 27–36 (1975)

  3. Foroughi-Wehr, B.; Wilson, H.M.; Mix, G.; Gaul, M.: Monohaploid plants from anthers of a dihaploid genotype of Solanum tuberosum L. Euphytica 26, 361–367 (1977)

  4. Hermsen, J.G.Th.; Verdenius, J.: Selection from Solanum tuberosum group ‘phureja’ of genotypes combining high-frequency haploid induction with homozygosity for embryo spot. Euphytica 22, 244–259 (1973).

  5. Irikura, Y.: Induction of haploid plants by anther culture in tuberbearing species and interspecific hybrids of Solanum. Potato Res. 18, 133–140 (1975)

  6. Jacobsen, E.: Cytological studies on diplandroid production in a dihaploid potato clone and its correlation with seed set in 4x X 2x crosses. Z. Pflanzenzüchtung 77, 10–15 (1976)

  7. Jacobsen, E.: Doubling dihaploid potato clones via leaf tissue culture. Z. Pflanzenzüchtung 80, 80–82 (1978)

  8. Jacobsen, E.: Haploid, diploid and tetraploid parthenogenesis in interspecific crosses between S. tuberosum interdihaploids and S. phureja Potato Res. In Press (1978)

  9. Melchers, G.: Genetik und Pflanzenzüchtung mit mikrobiologischen Methoden. Planta med. Suppl., 5–34 (1975)

  10. Murashige, T.; Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Pl. 15, 473–497 (1962)

  11. Nitzsche, W.; Wenzel, G.: Haploids in plant breeding. Berlin and Hamburg: Parey 1977

  12. Ross, H.; Jacobsen, E.: Beobachtungen an Nachkommenschaften aus Kreuzungen zwischen dihaploiden und tetraploiden Kartoffelformen; Samenzahl, Ploidiestufen, sowie Spaltungsverhältnisse des Gens für extreme Resistenz gegen das X-Virus (Rxacl). Z. Pflanzenzüchtung 76, 265–280 (1976)

  13. Simon, P.W.; Peloquin, S.J.: The influences of paternal species on the origin of callus in anther culture of Solanum hybrids Theor. Appl. Genet. 50, 53–56 (1977)

  14. Sopory, S.K.; Rogan, P.G.: Induction of pollen divisions and embryoid formation in anther cultures of some dihaploid clones of Solanum tuberosum. Z. Pflanzenphysiol. 80, 77–80 (1976)

  15. Sopory, S.K.; Jacobsen, E.; Wenzel, G.: In vitro production of monohaploid embryoids and plantlets in cultured anthers of Solanum tuberosum. Submitted (1977)

  16. Stegemann, M.; Loeschcke, V.: Index of European potato varieties. Mitteilungen aus der Biologischen Bundesanstalt für Landund Forstwirtschaft, Berlin-Dahlem 168 (1976)

  17. Tjio, J.H.; Levan, A.: The use of oxyquinoline in chromosome analysis. An. Estacion exp. Aula Dei 2 (21–64) (1950)

  18. Wenzel, G.; Hoffmann, F.; Thomas, E.: Increased induction and chromosome doubling of androgenetic haploid rye. Theor. Appl. Genet. In Press (1977)

Download references

Author information

Additional information

On leave from Jawaharal Nehru University, New Delhi (India)

Communicated by G. Melchers

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Jacobsen, E., Sopory, S.K. The influence and possible recombination of genotypes on the production of microspore embryoids in anther cultures of Solanum tuberosum and dihaploid hybrids. Theoret. Appl. Genetics 52, 119–123 (1978).

Download citation

Key words

  • Potato
  • Anther culture
  • Monohaploid embryoids
  • Genetic marker
  • Recombination