Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

An Haemophilus influenzae mutant which inhibits the growth of HP1c1 phage

  • 32 Accesses

  • 2 Citations

Summary

A strain of Haemophilus influenzae, called hpm - inhibits the growth of phage HP1c1 but not S2. This inhibition is overcome by HP1c1ph mutants. Phage HP1c1 adsorbs normally to hpm - cells but only a small fraction of infected cells produce phage with a normal burst size or become lysogenic. When hpm - strains lysogenic for HP1c1 are induced, 100% of the cells yield phage. There is no degradation of phage DNA after infection of hpm - cells and HP1c1 can normally grow when its DNA is introduced into hpm - by transfection. The most probable explanation is that in hpm - cells the penetration of phage DNA is blocked. The hpm - property behaves as as unstable mutation.

This is a preview of subscription content, log in to check access.

References

  1. Adams, M. H.: Bacteriophages. New York: Interscience Publishers 1959

  2. Arber, W.: DNA modification and restriction. Progr. Nucleic Acid Res. molec. Biol. 14, 1–37 (1947)

  3. Barnhart, B. J., Cox, S. H.: DNA replication of induced prophage in H. influenzae. J. Virol. 12, 165–176 (1973)

  4. Bendler, J. W., Goodgal, S. H.: Prophage S2 mutants in Haemophilus influenzae: a technique for their production and isolation. Science 162, 464–465 (1968)

  5. Boling, M. E., Allison, D. P., Setlow, J. K.: Bacteriophage of Haemophilus influenzae. III. Morphology, DNA homology and immunity properties of HP1c1, S2 and the defective bacteriophage from strain Rd. J. Virol. 11, 585–591 (1973)

  6. Boling, M. E., Setlow, J. K.: Marker rescue in Haemophilus influenzae. J. Virol. (in press)

  7. Coppo, A., Manzi, A., Pulitzer, J. F., Takahashi, H.: Abortive bacteriophage T4 head assembly in mutants of Escherichia coli. J. molec. Biol. 76, 61–87 (1973)

  8. Georgopoulos, C. P., Hendrix, R. W., Casjens, S. R., Kaiser, A. D.: Host participation in bacteriophage lambda assembly. J. molec. Biol. 76, 45–60 (1973)

  9. Glover, S. W., Piekarowicz, A.: Host specificity of DNA in Haemophilus influenzae: restriction and modification in strain Rd. Biochem. biophys. Res. Commun. 46, 1610–1618 (1972)

  10. Goodgal, S. H., Herriot, R. M.: Studies on transformation of Haemophilus influenzae. I. Competence. J. gen. Physio. 44, 1201–1227 (1961)

  11. Gromkova, R., Bendler, J., Goodgal, S. H.: A study of restriction and modification of bacteriophage S2 in Haemophilus influenzae. J. Bact. 114, 1151–1157 (1973)

  12. Harm, W., Ruppert, C. S.: Infection of transformable cells of Haemophilus influenzae by bacteriophage and bacteriphage DNA. Z. Vererbungsl. 94, 336–348 (1963)

  13. Holland, J. B., Threlefall, E. J., Holland, E. M., Darby, V., Samson, A. C. R.: Mutants of Escherichia coli with altered surface properties which are refractory to colicin E2, sensitive to ultraviolet light and which can also show recombination deficiency, abortive growth of bacteriophage lambda and filament formation. J. gen. Microbiol. 62, 371–382 (1970)

  14. Kerszman, G., Glover, S. W., Aronovitch, J.: The restriction of bacteriophage lambda in Escherichia coli strain W. J. gen. Microbiol. 1, 333–347 (1967)

  15. Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3, 208–218 (1961)

  16. Piekarowicz, A., Glover, S. W.: Host specificity of DNA in Haemophilus influenzae: the two restriction and modification systems in strain Ra. Molec. gen. Genet. 116, 11–25 (1972)

  17. Piekarowicz, A., Kauc, L., Glover, S. W.: Host specificity of DNA in Haemophilus influenzae: The restriction and modification systems in strains Rb and Rf. J. gen. Microbiol. 81, 391–403 (1974)

  18. Piekarowicz, A., Brzeziński, R., Kauc, L.: Host specificity of DNA in Haemophilus influenzae: The in vivo action of the restriction endonucleases on phage and bacterial DNA. Acta microbiol. pol. 7, 51–65 (1975)

  19. Scandella, D., Arber, W.: An Escherichia coli mutant which inhibits the injection of phage lambda DNA. Virology 58, 504–513 (1974)

  20. Shinozawa, T.: A mutant of Escherichia coli K12 unable to support the multiplication of bacteriophage BF23. Virology 54, 427–440 (1973)

  21. Spencer, H. T., Herriott, R. M.: Development of competence of Haemophilus influenzae. J. Bact. 90, 911–920 (1965)

  22. Stuy, J. H.: Phage resistance in Haemophilus influenzae. Biochem. biophys. Res. Commun. 33, 682–687 (1968)

Download references

Author information

Additional information

Communicated by W. Arber

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Jabłońska, E., Kauc, L. & Piekarowicz, A. An Haemophilus influenzae mutant which inhibits the growth of HP1c1 phage. Molec. Gen. Genet. 139, 157–166 (1975). https://doi.org/10.1007/BF00264695

Download citation

Keywords

  • Infected Cell
  • Haemophilus Influenzae
  • Cell Yield
  • Probable Explanation
  • Burst Size