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Stöchiometrische probleme der quantitativen fastgreen-zytophotometrie bei der histonbestimmung

Stoechiometrical problems of quantitative cytophotometry of fastgreen staining for histone proteins

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Summary

  1. 1.

    Nuclei of the different types of granulocytopoietic cells from normal human bone marrow smears were stained with Fastgreen FCF and their dye content was measured cytophotometrically.

  2. 2.

    The very immature cell types (myeloblasts and promyelocytes) showed a high percentage of unstained nuclei or nuclei with a dye content too faint to be measured. More mature stages showed increasing Fastgreen values which were highest in mature polymorphonuclear leukocytes.

  3. 3.

    The changing Fastgreen dye content of nuclei from different cell types—stained specifically for nuclear histone proteins—must be interpreted as varying stainability of histone proteins depending upon the functional state of chromatin. It does not indicate an altering histone content of the nuclei.

Zusammenfassung

  1. 1.

    An Knochenmarksausstrichen eines gesunden Menschen wurde die Fastgreen-Färbbarkeit der Kerne der verschiedenen Granulozytopoesezellen zytophotometrisch untersucht.

  2. 2.

    In den sehr unreifen Myeloblasten und Promyelozyten ist eine Fastgreen-Färbbarkeit der meisten Kerne nicht oder in nicht meßbarer Stärke vorhanden. Am stärksten ist die Fastgreen-Färbbarkeit der Kerne reifer Granulozyten.

  3. 3.

    Die bei verschiedenen Zellarten verschieden starke Anfärbung der Kerne mit Fastgreen bei pH 8,1, die für Histonproteine spezifisch sein soll, wird nicht als Ausdruck einer Histonmengenänderung, sondern als funktionsabhängige Änderung der Fastgreenkapazität der Histonproteine interpretiert.

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Noeske, K. Stöchiometrische probleme der quantitativen fastgreen-zytophotometrie bei der histonbestimmung. Histochemie 27, 243–252 (1971). https://doi.org/10.1007/BF00264396

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