Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture

  • 64 Accesses

  • 13 Citations


The oxidation of catechol, an intermediate in benzene catabolism, was studied using transient variations in dissolved oxygen tension (DOT) when a succinate limited steady state culture of Pseudomonas putida ML2 was perturbed with a pulse of another substrate. A model was developed and tested for the effect of fluctuations in oxidizing enzyme activity on DOT. It was found that the rate of induction of catechol oxidizing enzymes was independent of dilution rate up to a relative growth rate μ/μmax of 0.75. Only at higher dilution rates was catabolite repression observed.

This is a preview of subscription content, log in to check access.



dissolved oxygen tension

K L a:

gas transfer coefficient


specific growth rate

μmax :

maximum specific growth rate

Ks :

substrate saturation constant


  1. Ashby RE, Harrison DEF (1980) Studies on the induction and turnover of citrate oxidizing capacity in Klebsiella aerogenes J Gen Microbiol 120:465–473

  2. Axcell BC, Geary PJ (1975) Purification and some properties of a soluble benzene oxidizing system from a strain of Pseudomonas. Biochem J 142:173–183

  3. Bird JA, Cain RB (1968) cis, cis-muconic acid, the product inducer of catechol 1,2-oxygenase in Pseudomona aeruginosa Biochem J 109:479–481

  4. Cohen-Bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by non sulphur purple bacteria. J Cell Comp Physiol 49:25–68

  5. Dagley S (1986) Biochemistry of aromatic hydrocarbon degradation in pseudomonads. In: Sokatch JR (ed), The bacteria, vol X. Academic Press, London pp 527–555

  6. Dean ACR (1972) Influence of environment on the control of enzyme synthesis. J Appl Chem Biotechnol 22:245–259

  7. Geary PJ, Mason JR, Joannou CL (1990) The benzene dioxygenase from Pseudomonas putida. Methods Enzymol 188:52–60

  8. Harayama S (1991) Induction kinetics of RNA and proteins in exponentially growing organisms. Biochem Biophys Res Commun 180:913–919

  9. Hess HH, Lees MB, Derr JE (1978) A linear Lowry-Folin assay for both water soluble and sodium dodecyl sulphate-solubilized proteins. Anal Biochem 85:295–300

  10. Higgins SJ, Mandelstam J (1972) Regulation of pathways degrading aromatic substrates in P. putida. Biochem J 126:901–906

  11. Mason JR, Cammack R (1992) The electron-transport proteins of hydroxylating bacterial dioxygenases. Annu Rev Microbiol 46: 277–305

  12. Mason JR, Geary PJ (1990) cis-1,2-dihydroxycyclohexa-3,5-diene (NAD) oxidoreductase (cis-benzene dihydrodiol dehydrogenase) from Pseudomonas putida. NCIB 12190. Methods Enzymol 188:134–137

  13. Matin A, Veldkamp H (1978) Physiological basis of the selective advantage of a Spirillum sp in a carbon-limited environment. J Gen Microbiol 105:187–197

  14. Pirt SJ (1975) Principles of microbe and cell cultivation. Blackwell, London

  15. Ray NG, Vieth WR, Venkatasubramanian K (1986) Active inducer transport and regulation of microbial enzyme biosynthesis in chemostat cultures. Ann NY Acad Sci 469:212–229

  16. Stevenson IL, Mandelstam J, (1965) Induction and multi-sensitive product repression in two converging pathways degrading aromatic substances in Pseudomonas fluorescens Biochem J 96: 354–362

  17. Tan H-M, Tang H-Y, Joannou CL, Abdel-Wahab NH, Mason JR (1993) The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase are unusual in codon usage and low in G+C content. Gene 130:33–39

  18. Toda K (1981) Induction and repression of enzymes in microbial culture. J Chem Technol Biotechnol 31:775–790

  19. Viliesid F, Lilly MD (1992) Influence of dissolved oxygen tension on the synthesis of catechol 1,2-dioxygenase by Pseudomonas putida. Enz Microbial Technol 14:561–565

  20. Zamanian M, Mason JR, (1987) Benzene dioxygenase in Pseudomonas putida. Subunit composition and immuno-cross-reactivity with other aromatic dioxygenases. Biochem J 244:611–616

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mason, J.R. The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture. Arch. Microbiol. 162, 57–62 (1994). https://doi.org/10.1007/BF00264373

Download citation

Key words

  • Pseudomonas
  • Benzene catabolism
  • Catechol dioxygenase
  • continuous culture
  • Catabolite repression