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Glucose metabolism by brown trout peripheral blood lymphocytes

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Abstract

Glucose metabolism in peripheral blood lymphocytes from the brown trout Salmo trutta has been studied. Glucose is taken up by means of a sodium-independent saturable process (K m=10.8 mmol·l-1), as well as by simple diffusion. Once within the cell, most of glucose is directed to lactate production through either the Embden-Meyerhof pathway or the hexose-monophosphate shunt. Rates of lactate formation are higher than rates of CO2 formation. Glutamine does not exert an effect on either glucose uptake or glucose metabolism. The present study provides information regarding the nature of energy sources for different cell types in salmonids.

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Abbreviations

3-OMG:

3-O-methyl glucose

EM:

Embden-Meyerhoff pathway

G6D:

glucose-6-phosphate dehydrogenase

HK:

hexokinase

HMS:

hexose monophosphate shunt

ICDH:

isocitrate dehydrogenase

K m :

apparent Michaelis constant

LDH:

lactate dehydrogenase

MCB:

modified Cortland buffer

PBL:

peripheral blood lymphocytes

PFK:

fructose-6-phosphate kinase

PK:

pyruvate kinase

RBC:

red blood cells

V max :

maximal rate of uptake

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Albi, J.L., Planas, J. & Sánchez, J. Glucose metabolism by brown trout peripheral blood lymphocytes. J Comp Physiol B 163, 118–122 (1993). https://doi.org/10.1007/BF00263596

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Key words

  • Phloretin-sensitive uptake
  • Glucose metabolism
  • Aerobic and anaerobic metabolism
  • Peripheral blood lymphocytes
  • Trout, Salmo trutta