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Establishment of three human renal cell carcinoma cell lines (SMKT-R-1, SMKT-R-2, and SMKT-R-3) and their characters

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Summary

We have established three new cell lines of human renal cell carcinoma (RCC), designated as SMKT-R-1, SMKT-R-2 and SMKT-R-3. These cell lines were derived from a primary lesion of the tumor or a tumor initially xenotransplanted in nude mice. These cell lines have maintained a stable growth in vitro for more than a year. They also exhibited characteristics showing a lack of contact inhibition of cells, colony formation in soft agarose and tumor formation in nude mice by a xenotranaplantation of cells, all of which suggested an epithelial origin. The tumors produced in nude mice by the innoculation of cell lines were demonstrated by light an electron microscopy tobe derived from RCC. The doubling time of these cell lines were 180.0 h, in SMKT-R-1, 56.4 h in SMKT-R-2 and 55.7 h in SMKT-R-3. The cell lines were aneuploid in their chromosomal analysis, and SMKT-R-2 and SMKT-R-3 also had three and two marker chromosomes, respectively. The different biological characters of these cell lines from the others so far established would be of benefit in the future study of human RCC.

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References

  1. 1.

    Bear A, Clayman RV, Elbers J, Limas C, Wang N, Stone K, Gehard R, Prigge W, Palmer J (1987) Characterization of two human cell lines (TK-10, TK-164) of renal cell cancer. Cancer Res 47:3856–3862

  2. 2.

    deKernion JB, Sarna G, Figlin RL, Lindner A, Smith RB (1983) The treatment of renal cell carcinoma with human leukocyte alphainterferon. J Urol 130:1063–1066

  3. 3.

    Goldrosen M, Huben R, Miller GA, Lewis DA, Ochi H, Sandberg AA, Pontes JE (1985) Characterization of a renal cell carcinoma cell line suitable as a target for immunological studies in vitro and in the nu/nu mouse. J Urol 134:1271–1275

  4. 4.

    Hön W, Schroeder FH (1978) Renal cell carcinoma: two new cell lines and serially transplantable nude mice tumor (NC 65) —preliminary report. Invest Urol 16:106–112, 1978

  5. 5.

    Jones DW (1967) Primary and metastatic epithelial tumors of the human kidney and bladder in tissue culture. Cancer 20:1893–1898

  6. 6.

    Katsuoka Y (1978) Functional and morphological observation of human renal cell carcinoma utilizing nude mouse and cell culture. Jpn J Urol 69:285–303

  7. 7.

    Kinouchi T, Kotake T, Mori Y, Abe T (1984) Human renal cell carcinoma: establishment and characterization of new cell line (OS-RC-2). In Vitro 21:195–199

  8. 8.

    Komatsubara S (1978) Establishment of a new cell line (NRC-12) derived from a human renal cell carcinoma and its characteristics. Jpn J Urol 69:1535–1542

  9. 9.

    Matsuda M, Osafune M, Nakano E, Kotake T, Sonaoda T, Watanabe S, Harada T, Okuchi T, Higashino K, Yamamura Y, Abe T (1979) Characterization of an established cell line from human renal carcinoma. Cancer Res 39:4694–4699

  10. 10.

    Matsuda M, Sakuramoto T, Moriyama M, Miura T, Shuin T, Kubota Y (1986) Human renal cell carcinoma: establishment of a new cell line (YCR-1) using soft agar culture technique. Jpn J Urol 77:653–658

  11. 11.

    Mostofi FK, Davis CCJ (1980) Pathology of tumor of the kidney. In: Javadpour N (ed) Cancer of the kindey. Thieme-Statton, New York, pp 15–31

  12. 12.

    Naito S, Kanamori T, Hisano S, Tanaka K, Momose S, Kamata N (1982) Human renal cell carcinoma: eatablishment and characterization of two new cell lines. J Urol 128:1117–1121

  13. 13.

    Naito K, Hisazumi H, Kanokogi M, Katoh M, Nakajima K, Tsukahara K, Kobayashi T, Kuroda K, Matsubara F (1983) Tissue culture cell lines established from urologic carcinomas. Jpn J Urol 73:1019–1031

  14. 14.

    Okada Y, Gohji K, Kamidono S, Tachiwaki O (1986) Eatablishment and characterization of a cell line (KO-RCC-1) from a human renal cell carcinoma. Jpn J Urol 77:780–785

  15. 15.

    Olver IN, Leavitt RD (1984) Chemotherapy and immunotherapy of disseminated renal cancer. In: Javadpour N (ed) Cancer of the kidney. Thieme-Stratton, New York, pp 109–120

  16. 16.

    Quesada JR, Swanson DA, Trindade A, Gutterman JU (1983) Renal carcinoma: antitumor effect of leukocyte interferon. Cancer Res 43:940–947

  17. 17.

    Shipley GD, Ham RG (1981) Improved medium and culture conditions for clonal growth with minimal serum protein and for enhanced serum free survival of Swiss 3T3 cells. In Vitro 17:656–670

  18. 18.

    Shrivastav S, Shrief Y, Day J, Reich CF, Bonar RA (1981) Establishment and characterization of a cell line (SS 78) from a human renal cell carcinoma. In Vitro 17:1117–1124

  19. 19.

    Sytkowski AJ, Richie JP, Bicknell KA (1983) New human renal cell carcinoma cell line established from a patients with erythrocytosis. Cancer Res 43:1414–1419

  20. 20.

    Weaver DJ, Michalski K, Miles J (1988) Cytogenetic analysis in renal cell carcinoma: correlation with tumor aggressiveness. Cancer Res 48:2887–2889

  21. 21.

    Williams RD, Elliott AY, Stein N, Fraly EE (1976) In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12:623–627

  22. 22.

    Yano H, Maruiwa M, Sigihara S, Eto K (1988) Establishment and characterization of a new human renal cell carcinoma cell line (KRC/Y). In Vitro 24:9–16

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Miyao, N., Tsukamoto, T. & Kumamoto, Y. Establishment of three human renal cell carcinoma cell lines (SMKT-R-1, SMKT-R-2, and SMKT-R-3) and their characters. Urol. Res. 17, 317–324 (1989). https://doi.org/10.1007/BF00262990

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Key words

  • Human
  • Renal cell carcinoma
  • Established cell lines
  • Epithelial