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Cyclopiazonic acid disturbs the regulation of cytosolic calcium when repetitive action potentials are evoked in Dionaea traps

Abstract

Evoking of action potentials (APs) in the trap of Dionaea muscipula Ellis at intervals shorter than 20 s caused a gradual decrease in the amplitude of the APs. At longer intervals the amplitude was constant. The calcium ionophore A23187 (1 μM) caused a considerable decrease of AP amplitude. Pretreatment of a segment of the Dionaea trap with cyclopiazonic acid (CPA), which is a specific inhibitor of the Ca2+-ATPase in the sarcoplasmic seticulum of animal cells and in ER vesicles isolated from plant cells, only slightly affected the amplitude when APs were evoked every 10 min; however, it caused a considerable decrease in the amplitude when the stimulation was repeated every 2 min. Assuming that APs increase the concentration of cytosolic Ca2+ and the amplitude of AP depends on the gradient of Ca2+ across the plasma membrane, the effect of CPA on the AP amplitude indicates that CPA inhibits the sequestration of Ca2+ in Dionaea cells.

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Abbreviations

AP:

action potential

[Ca2+]c:

free Ca2+ concentration in the cytosol

CPA:

cyclopiazonic acid

DMSO:

dimethylsulfoxide

PM:

plasma membrane

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Author information

Correspondence to Andreas Sievers.

Additional information

This work was supported within the project AGRAVIS by Deutsche Agentur für Raumfahrtangelegenheiten, DARA, Bonn, and Ministerium für Wissenschaft und Forschung, Düsseldorf, to A.S., and by Alexander von Humboldt-Stiftung to K.T. We thank Dr. Sandra K. Hillman, University of Glasgow, UK, for correcting the manuscript and Dipl.-Ing. P. Blasczyak for help with the electronics.

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Trebacz, K., Busch, M.B., Hejnowicz, Z. et al. Cyclopiazonic acid disturbs the regulation of cytosolic calcium when repetitive action potentials are evoked in Dionaea traps. Planta 198, 623–626 (1996). https://doi.org/10.1007/BF00262650

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Key words

  • Action potential
  • Cyclopiazonic acid
  • Cytosolic calcium
  • Dionaea (action potential, calcium)