Evoking of action potentials (APs) in the trap of Dionaea muscipula Ellis at intervals shorter than 20 s caused a gradual decrease in the amplitude of the APs. At longer intervals the amplitude was constant. The calcium ionophore A23187 (1 μM) caused a considerable decrease of AP amplitude. Pretreatment of a segment of the Dionaea trap with cyclopiazonic acid (CPA), which is a specific inhibitor of the Ca2+-ATPase in the sarcoplasmic seticulum of animal cells and in ER vesicles isolated from plant cells, only slightly affected the amplitude when APs were evoked every 10 min; however, it caused a considerable decrease in the amplitude when the stimulation was repeated every 2 min. Assuming that APs increase the concentration of cytosolic Ca2+ and the amplitude of AP depends on the gradient of Ca2+ across the plasma membrane, the effect of CPA on the AP amplitude indicates that CPA inhibits the sequestration of Ca2+ in Dionaea cells.
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free Ca2+ concentration in the cytosol
Brown WH (1916) The mechanism of movement and the duration of the effect of stimulations in the leaves of Dionaea. Amer J Bot 3: 68–90
Busch MB, Sievers A (1993) Membrane traffic from the endoplasmic reticulum to the Golgi apparatus is disturbed by an inhibitor of the Ca2+-ATPase in the ER. Protoplasma 177: 23–31
Chanson A (1993) Active transport of proton and calcium in higher plant cells. Plant Physiol Biochem 31: 943–955
Demaurex N, Lew DP, Krause K-H (1992) Cyclopiazonic acid depletes intracellular Ca2+ stores and activates an influx pathway for divalent cations in HL-60 cells. J Biol Chem 267: 2318–2324
Evans DI, Briars S-A, Williams EE (1991) Active calcium transport by plant cell membrane. J Exp Bot 42: 285–303
Gilroy S, Fricker MD, Read ND, Trewavas AJ (1991) Role of calcium in signal transduction in Commelina guard cells. Plant Cell 3: 333–344
Felle HH, Tretyn A, Wagner G (1992) The role of plasma-membrane Ca2+ -ATPase in Ca2+ homeostasis in Sinapis alba root hairs. Planta 188: 306–313
Goeger DE, Riley RT, Dorner JW, Cole RJ (1988) Cyclopiazonic acid inhibition of the Ca2+-transport ATPase in rat skeletal muscle sarcoplasmic reticulum vesicles. Biochem Pharmacol 37: 978–981
Hodick D, Sievers A (1988) The action potential of Dionaea muscipula Ellis. Planta 174: 8–18
Hsieh W, Pierce WS, Sze H (1991) Calcium-pumping ATPases in vesicles from carrot cells. Stimulation by calmodulin or phosphatidylserine and formation of a 120 kilodalton phosphoenzyme. Plant Physiol 97: 1535–1544
Kikuyama M, Shimada K, Hiramoto Y (1993) Cessation of cytoplasmic streaming follows an increase of cytoplasmic Ca2+ during action potential in Nitella. Protoplasma 174: 142–146
Liß H, Weiler EW (1994) Ion-translocating ATPases in tendrils of Bryonia dioica Jacq. Planta 194: 169–180
Seidler NW, Jona I, Vegh M, Martonosi A (1989) Cyclopiazonic acid is a specific inhibitor of the Ca2+-ATPase of sarcoplasmic reticulum. J Biol Chem 264: 17816–17823
Sibaoka I (1966) Action potentials in plant organs. Symp Soc Exp Biol 20: 49–74
Sievers A, Busch MB (1992) An inhibitor of the Ca2+-ATPases in the sarcoplasmic and endoplasmic reticula inhibits transduction of the gravity stimulus in cress roots. Planta 188: 619–622
Thomson LJ, Hall JL, Williams LE (1994) A study of the effect of inhibitors of the animal sarcoplasmic/endoplasmic reticulum-type calcium pumps on the primary Ca2+-ATPases of red beet. Plant Physiol 104: 1295–1300
Trebacz K, Simonis W, Schönknecht G (1994) Cytoplasmic Ca2+, K+, Cl− and NO3-activities in the liverwort Conocephalum conicum L. at rest and during action potentials. Plant Physiol 106: 1073–1084
Williamson RE, Ashley CC (1982) Free Ca2+ and cytoplasmic streaming in the alga Chara. Nature 296: 647–650
This work was supported within the project AGRAVIS by Deutsche Agentur für Raumfahrtangelegenheiten, DARA, Bonn, and Ministerium für Wissenschaft und Forschung, Düsseldorf, to A.S., and by Alexander von Humboldt-Stiftung to K.T. We thank Dr. Sandra K. Hillman, University of Glasgow, UK, for correcting the manuscript and Dipl.-Ing. P. Blasczyak for help with the electronics.
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Trebacz, K., Busch, M.B., Hejnowicz, Z. et al. Cyclopiazonic acid disturbs the regulation of cytosolic calcium when repetitive action potentials are evoked in Dionaea traps. Planta 198, 623–626 (1996). https://doi.org/10.1007/BF00262650
- Action potential
- Cyclopiazonic acid
- Cytosolic calcium
- Dionaea (action potential, calcium)