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9-β-D-Arabinofuranosyl-2-fluoroadenine 5′-monophosphate pharmacokinetics in plasma and tumor cells of patients with relapsed leukemia and lymphoma

Summary

The pharmacokinetics of 9-β-D-arabinofuranosyl-2-fluoroadenine (F-ara-A) in plasma and its biologically active 5′-triphosphate (F-ara-ATP) in leukemic cells obtained from the peripheral blood and bone marrow was evaluated in patients with hematologic malignancies subsequent to the first dose of 20–125 mg/m2 per day for 5 days of F-ara-A 5′-monophosphate (F-ara-AMP) administered as an IV bolus over 30 min. The terminal half-lives of elimination of both F-ara-A (8 h) in plasma and intracellular F-ara-ATP (15 h) were not dependent upon the dose of F-ara-AMP. The area under the concentration x time curves for F-ara-A and F-ara-ATP, on the other hand, were increased in proportion to the prodrug dose. There was a high correlation between F-ara-ATP levels in circulating leukemic cells and those in bone marrow cells aspirated at the same time. DNA-synthetic capacity of leukemic cells was inversely related to the associated F-ara-ATP concentration. A linear trend was noted when F-ara-ATP levels in pretreatment peripheral blood leukemic cells incubated with F-ara-A in vitro were compared with the amount of F-ara-A that was incorporated into nucleic acids. Finally, F-ara-ATP concentrations were three times higher in bone marrow cells from patients with lymphomatous bone marrow involvement than from those without evidence of marrow disease.

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Author information

Correspondence to Lynn Danhauser.

Additional information

Supported in part by grants CA28153 and CA32839 from the National Cancer Institute, United States Department of Health and Human Services

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Danhauser, L., Plunkett, W., Keating, M. et al. 9-β-D-Arabinofuranosyl-2-fluoroadenine 5′-monophosphate pharmacokinetics in plasma and tumor cells of patients with relapsed leukemia and lymphoma. Cancer Chemother. Pharmacol. 18, 145–152 (1986). https://doi.org/10.1007/BF00262285

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Keywords

  • Bone Marrow
  • Lymphoma
  • Leukemic Cell
  • Triphosphate
  • Bone Marrow Cell