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Lignin and extractives derived inhibitors in the 2,3-butanediol fermentation of mannose-rich prehydrolysates

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Mannose, the predominant sugar in southern pine water prehydrolysates, has been fermented to 2,3-butanediol by Klebsiella pneumoniae AU-1-d3. Lignin derivatives and extractives soluble in the water prehydrolysates, however, hindered the butanediol fermentation. Treatments with sequential lime-sulfuric acid or mixed bed ion resins facilitated the butanediol fermentation of the water prehydrolysates. Fermentation inhibitors derived from southern pine lignin and extractives were identified.

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  1. Beck MJ, Strickland RC (1984) Fermentation of acid-hydrolyzed hardwood hemicellulose to ethanol. In: Proceedings of Tappi Research and Development Conference, Tappi, Atlanta, GA, pp 55–58

  2. Borchardt LG, Piper CV (1970) A gas chromatographic method for carbohydrates as alditol-acetates. Tappi 53 (2):257–260

  3. Browning BL (1967) Methods of Wood Chemistry, Vol. 2, Interscience Publishers, New York, pp 752–753

  4. Bunau G, Schade G, Gollnick K (1967) Massenspektrometrische Untersuchungen von Terpenen. I. Monoterpenaldehyde und-ketone. Z Anal Chem 227:173–187

  5. Casebier RL, Hamilton JK, Hergert HL (1969) Chemistry and mechanism of water prehydrolysis on southern pine wood. Tappi 52 (2):2369–2377

  6. Detroy RW, Cunningham RL, Bothast RJ, Bagby MO, Herman A (1982) Bioconversion of wheat straw cellulose/hemicellulose to ethanol by Saccharomyces uvarum and Pachysolen tannophilus. Biotechnol Bioeng 24:1105–1113

  7. Ekman R (1979) Analysis of the nonvolatile extractives in Norway spruce sapwood and heartwood. Acta Academiae Aboensis, Ser B 39 (4):1–20

  8. Goldschmid O (1955) Aqueous hydrolysis of lignin. I. Paper chromatographic separation of monomeric lignin degradation products. Tappi 38 (12):728–732

  9. Higuchi T, Tanahashi M, Sato A (1972) Acidolysis of bamboo lignin. I. Gas-liquid chromatography and mass spectrometry of acidolysis monomers. Mokuzai Gakkaishi 18 (4):183–189

  10. Holmbom B, Avela E (1971) Studies on tall oil from pine and birch. 1. Composition of fatty and resin acids in sulfate soaps and in crude tall oils. Acta Academiae Aboensis, Ser B 31 (13):1–14

  11. Jansen NB, Tsao GT (1983) Bioconversion of pentoses to 2,3-butanediol by Klebsiella pneumoniae. In: Adv Biochem Eng 27:85–99

  12. Jansen NB, Flickinger MC, Tsao GT (1984) Production of 2,3-butanediol from d-xylose by Klebsiella oxytoca ATCC 8724. Biotechnol Bioeng 26:362–369

  13. Jeffries TW (1983) Utilization of xylose by bacteria, yeast, and fungi. In: Fiechter A (ed) Advances in Biochemical Engineering/Biotechnology, Vol. 27. Springer-Verlag, New York, pp 1–32

  14. Lapierre C, Ralando C, Monties B (1983) Characterization of poplar lignins acidolysis products: capillary gas-liquid and liquid-liquid chromatography of monomeric compounds. Holzforschung 37 (4):189–198

  15. Lhuguenot JC, Maume BF, Baron C (1971) Gas chromatographic and mass spectrometric study of vanillin related compounds. Chromatographia 4:204–208

  16. Long SK, Patrick R (1963) The present status of the 2,3-butylene glycol fermentation. In: Umbriet WW (ed) Advances in applied microbiology. Vol. 5. Acacemic Press, New York, pp 135–155

  17. Maddox IS, Murray AE (1983) Production of n-butanol by fermentation of wood hydrolysate. Biotechnol Lett 5 (3):175–178

  18. Odham G, Stenhagen E (1972) Fatty acids. In: Waller GR (ed) Biochemical applications of mass spectrometry. Wiley-Interscience, New York, pp 211–249

  19. Rydholm SA (1965) Pulping processes, Interscience Publishers, New York, pp 657–658

  20. Sablayrolles JM, Goma G (1984) Butanediol production by Aerobacter aerogenes NRRL B199: Effect of initial substrate concentration and aeration agitation. Biotechnol Bioeng 26:148–155

  21. Sears KD, Beelik A, Casebier RL, Engen RJ, Hamilton JK, Hergert HL (1971) Southern pine prehydrolyzates: characterization of polysaccharides and lignin fragments. J Polymer Sci Part C 36:425–443

  22. Stanek KD (1958) A study of the low-molecular weight phenols formed upon the hydrolysis of aspenwood. Tappi 41 (10):601–609

  23. Stone JE, Scallan AM (1968) The effect of component removal upon the porous structure of the cell wall of wood. Pulp and Paper Mag Can 69 (9):T288-T293

  24. Sydow E (1963) Mass spectrometry of terpenes. II. Monoterpene alcohols. Acta. Chem Scand 17 (9):2504–2512

  25. Veeraraghavan S, Lee YY, Chambers RP, McCaskey TA (1980) Ethanol-butanediol fermentation of xylose. In: Weetall HH, Royer GP (eds) Enzyme Engineering. Vol. 5. Plenum Publishing Corporation, New York, pp 171–173

  26. Yu EKC, Saddler JN (1982) Power solvent production by Klebsiella pneumoniae grown on sugars present in wood hemicellulose. Biotechnol Lett 4:121–126

  27. Zinkel DF, Zank LC, Wesolowski MF (1971) Diterpene resin acids, USDA, Forest Service, Forest Products Laboratory, Madison, Wis., p C24

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Correspondence to Ai V. Tran.

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Tran, A.V., Chambers, R.P. Lignin and extractives derived inhibitors in the 2,3-butanediol fermentation of mannose-rich prehydrolysates. Appl Microbiol Biotechnol 23, 191–197 (1986). https://doi.org/10.1007/BF00261912

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  • Sugar
  • Fermentation
  • Lignin
  • Mannose
  • Klebsiella