Cellular proliferation in skeletal muscle was measured throughout the first 4 weeks after denervation. Twenty four mice had one leg denervated, and 4 groups of 6 of these mice were injected with tritiated thymidine once daily for 7 days, either during the first, second, third or fourth week after denervation. Autoradiographic labelling of muscle and connective tissue nuclei in denervated muscles was compared with innervated muscles from the opposite innervated legs of the same mice. Labelling of connective tissue and muscle (myonuclear and satellite cell) nuclei was significantly higher in denervated muscles, compared with innervated muscles on the unoperated side. There were no significant differences among labelling of nuclei in muscles denervated for 1, 2, 3 or 4 weeks. However, connective tissue labelling after 1 week of denervation was significantly higher than at later times. This study shows that nuclei of muscle and connective tissue cells proliferate and turnover at high levels for at least one month after denervation.
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Aloisi M, Mussini I, Schiaffino S (1973) Activation of muscle nuclei in denervation and hypertrophy. In: Kakulus BA (ed) Basic Research in Myology. Excerpta Medica. Amsterdam, pp 338–342
McGeachie J (1985) The fate of proliferating cells in skeletal muscle after denervation or tenotomy: An autoradiographic study. Neuroscience 15:499–506
McGeachie J, Allbrook D (1978) Cell proliferation in skeletal muscle following denervation or tenotomy: A series of autoradiographic studies. Cell Tissue Res 193:259–267
McGeachie JK, Grounds MD (1987) Initiation and duration of muscle precursor replication after mild and severe skeletal muscle injury in mice: an autoradiographic study. Cell Tissue Res 248:125–130
Murray MA, Robbins N (1982a) Cell proliferation in denervated muscle: time course, distribution and relation to disuse. Neuroscience 7:1817–1822
Murray MA, Robbins N (1982b) Cell proliferation in denervated muscle: identity and origin of dividing cells. Neuroscience 7:1823–1834
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McGeachie, J.K. Sustained cell proliferation in denervated skeletal muscle of mice. Cell Tissue Res. 257, 455–457 (1989). https://doi.org/10.1007/BF00261848
- Skeletal muscle
- Cell proliferation
- Tritiated thymidine
- vMouse (BALBc)