Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Thomsen-Friedenreich antigen in bladder tumors as detected by specific antibody: A possible marker of recurrence

  • 18 Accesses

  • 14 Citations


TUR specimens of non-invasive transitional-cell carcinomas were examined for their expression of Thomsen-Friedenreich (T) antigen by indirect immunofluorescence staining using rabbit IgG antibody which was raised with desialated glycophorin. Nine (45%) out of 20 tumors of low grade (Grade I and II), and 5 (56%) of 9 tumors of high grade (III) were diffusely stained with anti-T (T-positive), whereas T-antigen in normal tissue was cryptic and stained only after neuraminidase treatment (cryptic T-positive). Of the T-negative tumors, 9 (45%) of the low grade but only one of the high grade tumors, were stained positively for the cryptic T-antigen. The rest of the tumors were devoid of the cryptic T-antigen. Eighty percent of both Tumors expressing T-antigen and those lacking the cryptic T-antigen recurred within two years. Recurrence was not influenced by initial histological grade.

This is a preview of subscription content, log in to check access.


  1. 1.

    Bigbee WL, Vanderlaan M, Fong SSN, Jensen RH (1983) Monoclonal antibodies specific for the M- and N-Forms of human glycophorin A. Mol Immunol 20:1353–1362

  2. 2.

    Coon JS, Weinstein RS, Summers JL (1982) Blood group precursor T-antigen expression in human urinary bladder carcinoma. Am J Clin Pathol 77:692–699

  3. 3.

    Lehman TP, Cooper HS, Mulholland SG (1984) Peanut lectin binding sites in transitional cell carcinoma of the urinary bladder. Cancer 53:272–277

  4. 4.

    Limas C, Lange P (1980) Altered reactivity for A, B, H antigens in transitional cell carcinomas of the urinary bladder. Cancer 46:1366–1373

  5. 5.

    Marchesi VT, Andrews EP (1971) Glycoproteins: Isolation from cell membranes with lithium diiodosalicylate. Science 174:1247–1248

  6. 6.

    Pereira MEA, Kabat EA, Lotan R, Sharon N. (1976) Immunochemical studies on the specificity of the peanut (Arachis hypogaea) agglutinin. Carbohydr Res 51:107–118

  7. 7.

    Springer GF, Desai PR (1974) Common precursors of human blood group MN specificities. Biochem Biophys Res Commun 61:420–425

  8. 8.

    Springer GF, Desai PR, Banatwala I (1974) Blood group MN specific substances and precursors in normal and malignant human breast tissues. Naturwissenschaften 61:457–458

  9. 9.

    Springer GF, Desai PR, Banatwala I (1975) Brief Communication: Blood group MN antigens and precursors in normal and malignant human breast glandular tissue. J Natl Cancer Inst 54:335–339

  10. 10.

    Springer GF, Desai PR (1975) Human blood-group MN and precursor specificities: structural and biological aspects. Carbohydr Res 40:183–192

  11. 11.

    Springer GF, Desai PR, Murthy MS, Tegtmeyer H, Scanlon EF (1979) Human carcinoma-associated precursor antigens of the blood group MN system and the host's immune responses to them. Prog Allergy 26:42–96

  12. 12.

    Springer GF, Desai PR (1981) Extent of desialation of blood group MM, NN, and MN antigens required for reactivity with human anti-T antibody and Arachis hypogaea lectin. J Biol Chem 257:2744–2746

  13. 13.

    Summers JL, Coon JS, Ward RM, Falor WH, Miller III AW, Weinstein RS (1983) Prognosis in carcinoma of the urinary bladder based upon tissue blood group ABH and Thomsen-Friedenreich antigen status and karyotype of the initial tumor. Cancer Res 43:934–939

  14. 14.

    Warren L (1959) The thiobarbituric acid assay of sialic acids J Biol Chem 234:1971–1975

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ohoka, H., Shinomiya, H., Yokoyama, M. et al. Thomsen-Friedenreich antigen in bladder tumors as detected by specific antibody: A possible marker of recurrence. Urol. Res. 13, 47–50 (1985). https://doi.org/10.1007/BF00261564

Download citation

Key words

  • T-antigen
  • Anti-T antibody
  • Urinary bladder cancer
  • Recurrence