The sequence of the PcnB protein of Escherichia coli, a protein required for copy number maintenance of ColE1-related plasmids, was compared with the PIR sequence database. Strong local similarities to the sequence of the E. coli protein tRNA nucleotidyltransferase were found. Since a substrate of the latter protein, tRNA, structurally resembles the RNAs that control ColE1 copy number we believe that we may have identified a region in PcnB that interacts with these RNAs. Consistent with this idea is our observation that PcnB is required for the replication of R1, a plasmid whose replication is also regulated by a small RNA.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Cudny H, Lupski JR, Godson GN, Deutscher MP (1986) Cloning, sequencing and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidlytransferase. J Biol Chem 261:6444–6449
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
Foulds J, Hilderman RH, Deutscher MP (1974) Mapping of the locus for Escherichia coli transfer ribonucleic acid nucleotidyltransferase. J Bacteriol 118:628–632
George DG, Barker WC, Hunt LT (1986) The protein identification resource (PIR). Nucleic Acids Res 14:11–16
Gerdes K, Rasmussen PB, Molin S (1986) Unique type of plasmid maintenance function: post-segregational killing of plasmid free cells. Proc Natl Acad Sci USA 83:3116–3120
Green PJ, Pines O, Inouye M (1986) The role of antisense RNA in gene regulation. Annu Rev Biochem 55:569–597
Larsen JEL, Gerdes K, Light J, Molin S (1984) Low-copy-number plasmid cloning vectors amplifiable by derepression of an inserted foreign promoter. Gene 28:45–54
Liu J, Parkinson JS (1989) Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control. J Bacteriol 171:1254–1261
Lopilato J, Bortner S, Beckwith J (1986) Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol Gen Genet 205:285–290
Lyall A, Hill C, Collins JF, Coulson AFW (1985) Implementation of inexact strin matching algorithms on the I.C.L. DAP. p 235–240. In: Feilmeier M, Joubert G, Schendel U (eds) Parallel computing 85, Elsevier Science Publ (N Holland)
March JB, Colloms MD, Hart-Davis D, Oliver IR, Masters M (1989) Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number. Mol Microbiol 3:903–910
Minden JS, Marians KJ (1985) Replication of pBR322 DNA in vitro with purified proteins. Requirement for topoisomerase I in the maintenance of template specificity. J Biol Chem 260:9316–9325
Molin S, Stougaard P, Uhlin BE, Gustafsson P, Nordström K (1979) Clustering of genes involved in replication, copy number control and stable maintenance of the resistance plasmid R1drd-19. J Bacteriol 138:70–79
Nordström K, Molin S, Light J (1984) Control of replication of bacterial plasmids: Genetics, molecular biology and physiology of the plasmid R1 system. Plasmid 12:71–90
Simons RW, Kleckner N (1988) Biological regulation by antisense RNA in prokaryotes. Annu Rev Genet 22:567–600
Smith TF, Waterman MS (1981) Comparison of bio-sequences. Adv Appl Math 26:787–793
Tomizawa J, Som J (1984) Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the rom protein. Cell 38:871–878
Uhlin BE, Nordström K (1977) R plasmid gene dosage effects in E. coli K-12: copy mutants of the R plasmid R1drd-19. Plasmid 1:1–7
Yavachen L, Ivanov I (1988) What does the homology between E. coli tRNAs and RNAs controlling ColE1 plasmid replication mean? J Theor Biol 131:235–241
Wagner EGH, Nordström K (1986) Structural analysis of an RNA molecule involved in the replication control of plasmid R1. Nucl Acids Res 14:2523–2538
Zuh L, Cudny H, Deutscher MP (1986) A mutation in Escherichia coli tRNA nucleotidyltransferase that affects only AMP incorporation is in a sequence often associated with nucleotide binding proteins. J Biol Chem 261:14875–14877
Communicated by D.J. Finnegan
About this article
Cite this article
Masters, M., March, J.B., Oliver, I.R. et al. A possible role for the pcnB gene product of Escherichia coli in modulating RNA:RNA interactions. Molec. Gen. Genet. 220, 341–344 (1990). https://doi.org/10.1007/BF00260507
- Plasmid copy number
- tRNA nucleotidyltransferase
- Replication control