Immobilization of the thermophilic bacterium Thermus aquaticus YT-1 has been studied using various entrapment techniques. Alginate, κ-carrageenan, agar, agarose and polyacrylamide were tested as supports during operation at 65°C at which the cells are known to produce protease when grown free in solution. Alginate showed toxic effects and no viability was observed after entrapment in Ca alginate or even after exposure of free living cells to sodium alginate. Polyacrylamide was observed to be the best support. Protease activity was closely related to the appearance of free cells in the medium.
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Kanasawud, P., Hjörleifsdottir, S., Holst, O. et al. Studies on immobilization of the thermophilic bacterium Thermus aquaticus YT-1 by entrapment in various matrices. Appl Microbiol Biotechnol 31, 228–233 (1989). https://doi.org/10.1007/BF00258400
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