Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Enrichment of acetate-, propionate-, and butyrate-degrading co-cultures from the biofilm of an anaerobic fluidized bed reactor

Summary

Scanning electron microphotographs from the biofilm of a pilot scale anaerobic fluid-ized-bed reactor fed with acetate, propionate, and butyrate as carbon sources showed a predominance of filamentous organisms resembling Methanothrix sp. which could be isolated as an al-most pure culture as well as a Methanosarcina strain. Three syntrophic cultures, enriched in the medium of Boone and Xun, contained four or five microscopically distinguishable microorganisms, among them Methanospirillum sp., Methanothrix sp., Methanosarcina sp., and rods of acetogenic bacteria degrading propionate or butyrate effectively.

This is a preview of subscription content, log in to check access.

References

  1. Ahring BK, Westermann P (1987) Thermophilic anaerobic degradation of butyrate by a butyrate-utilizing bacterium in coculture and triculture with methanogenic bacteria. Appl Environ Microbiol 53:429–433

  2. Boone DR, Bryant MP (1980) Propionate-degrading bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from methanogenic ecosystems. Appl Environ Microbiol 40:626–632

  3. Boone DR, Xun L (1987) Effect of pH, temperature, and nutrients on propionate degradation by a methanogenic enrichment culture. Appl Environ Microbiol 53:1589–1592

  4. Brune G, Schobert SM, Sahm H (1982) Anaerobic treatment of an industrial waste water containing acetic acid, furfural and sulphite. Process Biochem 17:20–35

  5. Ehlinger F, Audic JM, Faup GM (1987) Relationship between the concentration of acetate in the feed and the composition of a biofilm in an aerobic filter. Environ Technol Lett 8:197–207

  6. Ferry JG, Smith PH, Wolfe RS (1974) Methanospirillum, a new genus of methanogenic bacteria and characterization of Methanospirillum hungatii sp. nov. Int J Syst Bacteriol 24:465–469

  7. Gorris LGM, Deursen JMA van, Drift C van der, Vogels GD (1988) Influence of waste water composition on biofilm development in laboratory methanogenic fluidized bed reactors. Appl Microbiol Biotechnol 29:95–102

  8. Gottschalk G, Andreesen JR, Hippe G (1981) The genus Clostridium. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The procaryotes, vol. 2. Springer, Berlin, Heidelberg, New York, pp 1767–1803

  9. Heijnen JJ (1984) Technik der anaeroben Abwasserreinigung. Chem-Ing Techn 56:526–532

  10. Hippe H, Caspari D, Fiebig K, Gottschalk G (1979) Utilization of trimethylamine and other N-methyl compounds for growth and methane formation by Methanosarcina barkeri. Proc Natl Acad Sci USA 76:494–498

  11. Huser BA, Wuhrmann K, Zehnder AJB (1982) Methanothrix soehngenii gen. nov. sp. nov., a new acetotrophic, non-hydrogen oxidizing methane bacterium. Arch Microbiol 132:1–9

  12. Jördening H-J, Pellegrini A, Buchholz K (1988) Der Einsatz von Fliessbettreaktoren zur anaeroben Reinigung von Zuckerfabriksabwässern. Zuckerindustrie 12:1033–1039

  13. McInerney MJ, Bryant MP, Pfennig N (1979) Anaerobic bacterium that degrades fatty acids in syntrophic association with methanogens. Arch Microbiol 122:129–135

  14. McInerney MJ, Roderick LM, Bryant MP (1981a) Syntrophic association of a butyrate-degrading bacterium and Methanosarcina enriched from bovine rumen fluid. Appl Environ Microbiol 41:826–828

  15. McInerney MJ, Bryant MP, Hespell RB, Costerton JW (1981b) Syntrophomonas wolfei gen. nov. sp. nov., an anaerobic, syntrophic, fatty acid-oxidizing bacterium. Appl Environ Microbiol 41:1029–1039

  16. Mucha H, Lingens F, Trösch W (1988) Conversion of propionate to acetate and methane by syntrophic consortia. Appl Microbiol Biotechnol 27:581–586

  17. Scherer PA, Bochem HP, Davis JD, White DC (1986) Flocculations in methanogens, a comparative study of Methanosarcina barkeri strains Jülich and Fusaro. Can J Microbiol 32:137–144

  18. Switzenbaum MS (1983) Anaerobic fixed film waste water treatment. Enzyme Microbiol Technol 5:242–250

  19. Ten Brummeler E, Hulshof Pol LW, Dolfing J, Lettinga G, Zehnder AJB (1985) Methanogenesis in an upflow anaerobic sludge blanket reactor at pH 6 on acetate-propionate mixture. Appl Environ Microbiol 49:1472–1477

  20. Wollersheim R, Jördening H-J, Diekman H (1988) Veränderung der Biomassezusammensetzung bei der Inbetriebnahme eines anaeroben Wirbelschichtreaktors. gwf-Wasser/Abwasser 129:759–764

  21. Zellner G, Winter J (1987) Analysis of a highly efficient methanogenic consortium producing biogas from whey. Syst Appl Microbiol 9:284–292

Download references

Author information

Correspondence to Regina Wollersheim.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wollersheim, R., Selz, A., Heppner, B. et al. Enrichment of acetate-, propionate-, and butyrate-degrading co-cultures from the biofilm of an anaerobic fluidized bed reactor. Appl Microbiol Biotechnol 31, 425–429 (1989). https://doi.org/10.1007/BF00257616

Download citation

Keywords

  • Propionate
  • Butyrate
  • Pure Culture
  • Pilot Scale
  • Methanosarcina