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Isolation and characterization of a derepressed mutant of Schwanniomyces castellii for amylase production

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Summary

The production of α-amylase and amyloglucosidase activity in the yeast Schwanniomyces castellii strain CBS 2863 is repressed in the presence of glucose. Mutants displaying increased amylase activity were obtained after treatment with UV light and screening for resistance to 2-deoxy-glucose. One mutant was found to exhibit derepressed amylase activity. Biosynthesis and the rate of excretion did not appear to be as highly sensitive to dissolved oxygen, pH and dilution rate as in the parental strain.

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Correspondence to G. Moulin.

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Boze, H., Guyot, J.B., Moulin, G. et al. Isolation and characterization of a derepressed mutant of Schwanniomyces castellii for amylase production. Appl Microbiol Biotechnol 31, 366–370 (1989). https://doi.org/10.1007/BF00257606

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Keywords

  • Oxygen
  • Glucose
  • Dissolve Oxygen
  • Amylase
  • Parental Strain