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99mTc-labelling of leucocytes with 99mTc-DPO: a complex developed for myocardial imaging

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The lipophilic 99mTc-DPO complex, developed as a myocardial imaging radiopharmaceutical, was used to label leucocytes. After an incubation of 0.1 ml 99mTc-DPO (8 μg DMPE*2HCl) with mixed leucocytes in plasma, the labelling efficiency was over 70%. During incubation in 5 ml plasma, a loss of activity was found between 20% (1 h) and 35% (3 h) caused by elution. Disturbances of cell viability could not be found with the help of the chemiluminescence test. The in vivo recovery was determined in three dogs and was 45%–50% (0.5 h), 30%–36% (1 h), and 18%–24% (3 h). Autologous 99mTc-DPO-leucocytes were used on seven patients with suspected osteomyelitis, there were four true negative and three true positive results. The target/nontarget ratio determined by ROI in the positive cases was 1.8 to 2.5 at 3 h after injection.

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  1. Becker W, Börner W (1988) 99mTc-HM PAO zur Markierung von Leukozyten und Thrombozyten. Besonderheiten und diagnostische Konsequenzen. Fortschr. Röntgenstr 148:69–74

  2. Danpure H-J, Osman S (1988) Optimum conditions for radiolabelling human granulocytes and mixed leucocytes with 111In-tropolonate. Eur J Nucl Med 13:537–542

  3. Joseph K, Damann V, Engeroff G, Grunner KR (1986) Markierung von Leukozyten mit 99mTc-Technetium-HM-PAO: Erste klinische Ergebnisse. Nuc Compact 17:277–283

  4. Kampf G, Muenze R, Knop G, Seifert S (1987) Evaluation of potential heart affine compounds by use of the isolated perfused rat heart. Eur J Nucl Med 13:410–415

  5. McAfee JG, Subramanian G, Gagne G, Schneider RF, Zapf-Longo C (1987) 99mTc-HM-PAO for leukocyte labeling-experimental comparison with 111In oxine in dogs. Eur J Nucl Med 13:353–357

  6. Mohnike W, Schmidt J, Uhlich F, Seifert S, Syhre R, Muenze R (1987) Klinische Ergebnisse zur Myokardperfusionsdiagnostik mit 99mTc-DPO, zubereitet aus Rotop-Myospect. Nuc Compact 18:184–188

  7. Peters AM, Danpure HJ, Osman S, Hawker RJ, Henderson BL, Hodgson HJ, Kelly JD Neirinckx RD, Lavender JP (1986) Clinical experience with 99mTc-hexamethylpropylene-amineoxime for labelling leucocytes and imaging inflammation. Lancet 2:946–949

  8. Schümichen C, Schölmerich J (1986) 99mTc-HM-PAO labelling of leucocytes for detection of inflammatory bowel disease. Nuc Compact 17:274–276

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Correspondence to G. Endert.

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Endert, G., Franke, U. & Kleinert, P. 99mTc-labelling of leucocytes with 99mTc-DPO: a complex developed for myocardial imaging. Eur J Nucl Med 15, 262–264 (1989). https://doi.org/10.1007/BF00257545

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Key words

  • Cell labelling
  • 99mTc-leucocytes
  • 99mTc complexes
  • 99mTc-DPO-leucocyte labelling