The behaviour and state of soil catalase, dehydrogenases, urease and proteases associated with different soil structural fractions were studied. Assays of the enzymatic sensitivity to pH variation, thermal stability and the calculation of kinetics constants of Michaelis were performed. The results indicated that catalase and urease activity in these soils seem to be of the same type, because the activities presented a similar behaviour in the soil fractions studied. However, their state appeared different in each group of soil units. Dehydrogenases showed a similar state and behaviour while proteases were in a different state and behaviour in each soil fraction.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Benesi A, McLaren AD (1976) A microenvironmental redox shift at a charged surface detected by papain activity. J Solid-Phase Biochem 1:27–32
Bergmeyer HU (1983) Methods of enzymatic analysis, vol III. In: BergmeyerHU (ed) Enzymes 1: oxidoreductases, transferases. Verlag Chemie, Weinheim
Bergmeyer HU (1984) Methods of enzymatic analysis, vol V. In: Bergmeyer HU (ed) Enzymes 3: peptidases, proteinases and their inhibitors. Verlag Chemie, Weinheim
Burns RG (1983) Extracellular enzyme-substrate interactions in soil. In: Slater JH, Whittenbury R, Wimpenny JWT (eds) Microbes in their natural environments. Symp 34 Soc Gen Microb. Cambridge University Press, Cambridge, pp 249–298
Chabas LJ (1969) Enzimologia. Editorial Cientifico-Médica, Barcelona
Engasser J, Horvath C (1975) Electrostatic effects on the kinetics of bound enzymes. Biochem J 145:431–435
Irving GC, Cosgrove DJ (1976) The kinetics of soil acid phosphatase. Soil Biol Biochem 8:335–340
Kruskal WH, Wallis WA (1952) Use of ranks in one criterion variance analysis. J Am Statist An 47:583–621
McLaren AD, Packer L (1970) Some aspects of enzyme reactions in heterogeneous systems. Adv Enzymol 33:245–308
McLaren AD, Pukite AH, Barshad I (1975) Isolation of humus with enzymatic activity from soil. Soil Sci 119:178–180
Nannipieri P, Ceccanti B, Cervelli S, Sequi P (1978) Stability and kinetic properties of humus-urease complexes. Soil Biol Biochem 10:143–147
Perez Mateos M, Gonzalez Carcedo S (1985) Effect of fractionation on location of enzyme activities in soil structural units. Biol Fertil Soils 1:153–159
Pettit NM, Smith AR, Freedman RB, Burns RG (1976) Soil urease: activity, stability and kinetic properties. Soil Biol Biochem 8:479–484
Skujins J (1978) History of abiontic soil enzyme research. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 1–33
Teorell L, Stenhagen M (1938) A solution as an universal buffer. Biochem Z 299:416
Zaborsky OR (1973) Immobilized enzymes. CRC, Cleveland, Ohio
About this article
Cite this article
Perez Mateos, M., Gonzalez Carcedo, S. Effect of fractionation on the enzymatic state and behaviour of enzyme activities in different structural soil units. Biol Fert Soils 4, 151–154 (1987). https://doi.org/10.1007/BF00256989
- pH activity curves
- Soil enzymes
- Structural soil units
- Thermal stability