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Effect of fractionation on the enzymatic state and behaviour of enzyme activities in different structural soil units

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Summary

The behaviour and state of soil catalase, dehydrogenases, urease and proteases associated with different soil structural fractions were studied. Assays of the enzymatic sensitivity to pH variation, thermal stability and the calculation of kinetics constants of Michaelis were performed. The results indicated that catalase and urease activity in these soils seem to be of the same type, because the activities presented a similar behaviour in the soil fractions studied. However, their state appeared different in each group of soil units. Dehydrogenases showed a similar state and behaviour while proteases were in a different state and behaviour in each soil fraction.

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References

  1. Benesi A, McLaren AD (1976) A microenvironmental redox shift at a charged surface detected by papain activity. J Solid-Phase Biochem 1:27–32

  2. Bergmeyer HU (1983) Methods of enzymatic analysis, vol III. In: BergmeyerHU (ed) Enzymes 1: oxidoreductases, transferases. Verlag Chemie, Weinheim

  3. Bergmeyer HU (1984) Methods of enzymatic analysis, vol V. In: Bergmeyer HU (ed) Enzymes 3: peptidases, proteinases and their inhibitors. Verlag Chemie, Weinheim

  4. Burns RG (1983) Extracellular enzyme-substrate interactions in soil. In: Slater JH, Whittenbury R, Wimpenny JWT (eds) Microbes in their natural environments. Symp 34 Soc Gen Microb. Cambridge University Press, Cambridge, pp 249–298

  5. Chabas LJ (1969) Enzimologia. Editorial Cientifico-Médica, Barcelona

  6. Engasser J, Horvath C (1975) Electrostatic effects on the kinetics of bound enzymes. Biochem J 145:431–435

  7. Irving GC, Cosgrove DJ (1976) The kinetics of soil acid phosphatase. Soil Biol Biochem 8:335–340

  8. Kruskal WH, Wallis WA (1952) Use of ranks in one criterion variance analysis. J Am Statist An 47:583–621

  9. McLaren AD, Packer L (1970) Some aspects of enzyme reactions in heterogeneous systems. Adv Enzymol 33:245–308

  10. McLaren AD, Pukite AH, Barshad I (1975) Isolation of humus with enzymatic activity from soil. Soil Sci 119:178–180

  11. Nannipieri P, Ceccanti B, Cervelli S, Sequi P (1978) Stability and kinetic properties of humus-urease complexes. Soil Biol Biochem 10:143–147

  12. Perez Mateos M, Gonzalez Carcedo S (1985) Effect of fractionation on location of enzyme activities in soil structural units. Biol Fertil Soils 1:153–159

  13. Pettit NM, Smith AR, Freedman RB, Burns RG (1976) Soil urease: activity, stability and kinetic properties. Soil Biol Biochem 8:479–484

  14. Skujins J (1978) History of abiontic soil enzyme research. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 1–33

  15. Teorell L, Stenhagen M (1938) A solution as an universal buffer. Biochem Z 299:416

  16. Zaborsky OR (1973) Immobilized enzymes. CRC, Cleveland, Ohio

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Correspondence to M. Perez Mateos.

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Perez Mateos, M., Gonzalez Carcedo, S. Effect of fractionation on the enzymatic state and behaviour of enzyme activities in different structural soil units. Biol Fert Soils 4, 151–154 (1987). https://doi.org/10.1007/BF00256989

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Key words

  • Kinetics
  • pH activity curves
  • Soil enzymes
  • Structural soil units
  • Thermal stability