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Deterioration and reactivation of beneficial rhizopseudomonads of barley (Hordeum vulgare)

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Summary

The beneficial effect of rhizopseudomonad strains on the growth of the barley cultivar Iban as a function of the storage time under laboratory conditions was evaluated. The beneficial rhizopseudomonad strains were preserved on slants of Modified King Medium B (MKB) at 28 °C and transferred every 2 weeks. After 6 months of storage most strains lost their beneficial effect on plant growth. Cold shock treatment enabled some strains to recover their beneficial effect on plant growth. Evidence is presented that there is a relationship between recovery of the stimulatory effect of certain rhizopseudomonad strains on plant growth and recovery of the capacity to colonize roots and to express antifungal activity.

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References

  1. Bhuvaneswari TV, Mills KK, Crist DK, Evans WR, Bauer WD (1983) Effects of culture age on symbiotic infectivity of Rhizobium japonicum. J Bacteriol 153:443–451

  2. Iswandi A, Bossier P, Vandenabeele J, Verstraete W (1987a) Relation between microbial activity and the effect of seed inoculation with the rhizopseudomonad strain 7NSK2 on plant growth. Biol Fertil Soils 3:147–151

  3. Iswandi A, Bossier P, Vandenabeele J, Verstraete W (1987b) Effect of seed inoculation with the rhizopseudomonad strain 7NSK2* on the root microbiota of maize and barley. Biol Fertil Soils 3:153–158

  4. Iswandi A, Bossier P, Vandenabeele J, Verstraete W (1987c) Influence of the inoculum density of the rhizopseudomonad strain 7NSK2 on the growth and the root microbial composition of maize (Zea mays) and barley (Hordeum vulgare). Biol Fertil Soils 4:121–125

  5. Kelman A (1953) The bacterial wilt caused by Pseudomonas solanacearum. N C Agric Exp Stn Tech Bull 99:1–194

  6. Kloepper JW, Schroth MN, Miller TD (1980) Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology 70:1078–1082

  7. Kole HK, Bose SK (1980) A note on the development of a technique for regeneration of a degenerated culture. J Appl Bacteriol 48:433–436

  8. Scher FM, Ziegle JS, Kloepper JW (1984) A method for assessing the root-colonizing capacity of bacteria on maize. Can J Microbiol 30:151–157

  9. Simon A, Rovira AD, Sands DC (1973) An improved selective medium for isolating fluorescent pseudomonads. J Appl Bacteriol 36:141–145

  10. Suslow TV, Schroth MN (1982) Rhizobacteria of sugarbeets: effects of seed application and root colonization on yield. Phytopathology 72:199–206

  11. Weller DM (1983) Colonization of wheat roots by a fluorescent pseudomonad suppressive to take-all. Phytopathology 73:1548–1553

  12. Weller DM, Cook RJ (1983) Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads. Phytopathology 73:463–469

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Correspondence to W. Verstraete.

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Iswandi, A., Bossier, P., Vandenabeele, J. et al. Deterioration and reactivation of beneficial rhizopseudomonads of barley (Hordeum vulgare). Biol Fert Soils 4, 125–128 (1987). https://doi.org/10.1007/BF00256985

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Key words

  • Rhizopseudomonads
  • Deterioration
  • Reactivation
  • Cold shock
  • Root colonization
  • Antifungal activity