Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effects of LH and oestrogen on the growth of feminising testicular tumours in mice


Testicular tumours in mice with testicular feminisation (tfm/y) are made up of masses of large Leydig and lipid-laden cells, surrounded by darkly-stained smaller Leydig cells. The activity of 3 β-hydroxysteroid dehydrogenase (3 β-HSDH) is more apparent in the small cells of the peripheral than in the larger cells of the central part of the tumour. Oestrogen alone can stimulate the incorporation of thymidine into deoxyribonucleic acid (DNA) by cultured cells but not the activity of 3β-HSDH. On the other hand, luteinizing hormone (LH) promotes activity of the enzyme, but not the synthesis of DNA. Together, they have a greater effect on DNA synthesis and enzyme activity than if present separately. It seems that in tfm/y mice gonadotrophin is necessary for the proper action of oestrogen in control of testicular tumour growth.

This is a preview of subscription content, log in to check access.


  1. 1.

    Attardi, B., Ohno, S.: Cytosol androgen receptor from kidney of normal and testicular feminized (Tfm) mice. Cell 2, 205 (1974)

  2. 2.

    Blackburn, W. R., Chung, K. W., Bullock, L. P., Bardin, C. W.: Testicular feminization in the mouse: Studies of Leydig cell structure and function. Biology of Reproduction 9, 9 (1973)

  3. 3.

    Bullock, L. P., Bardin, C. W.: Androgen receptors in mouse kidney: A study of male, female and androgen-insensitive (tfm/y) mice. Endocrinology 94, 746 (1974)

  4. 4.

    Chung, K. W.: Effects of tropic hormones on ultrastructure and synthesis of androgens in adrenals of male pseudohermaphrodite rats. Cell and Tissue Research 189, 469 (1978)

  5. 5.

    Chung, K. W.: Spontaneous testicular neoplasm in mice with testicular feminization. Cell and Tissue Research (in press)

  6. 6.

    Daino, J. A., Tcherkoff, V., Avahavahn, T. H., Ober, W. B.: Leydig cell tumor of the testis in a male pseudohermaphrodite with testicular feminization. New York State Journal of Medicine 63, 2258 (1963)

  7. 7.

    Goldstein, J. L., Wilson, J. D.: Studies on the pathogenesis of the pseudohermaphroditism in the mouse with testicular feminization. Journal of Clinical Investigation 51, 1647 (1972)

  8. 8.

    Huseby, R. A.: Estrogen-induced Leydig cell tumor in the mouse. A model system for the study of carcinogenesis and hormone dependency. Journal of Toxicology and Environmental Helath (Suppl) 1, 177 (1976)

  9. 9.

    Jacobs, B. B., Huseby, R. A.: Transplantable Leydig cell tumors in Fisher rats: Hormone responsibility and hormone production. Journal of National Cancer Institute 41, 1141 (1968)

  10. 10.

    Jull, J. W., McClellan, D., Coupey, S.: Steroid metabolism by an oestrogen-dependent interstitial-cell tumour of a rat testis. Journal of Endocrinology 59, 7 (1973)

  11. 11.

    Kurland, G., Christensen, A. K., Huseby, R. A.: Changes in fine structure accompanying estrogen induced tumorigenesis of Leydig cells in the mouse testis. Cancer Research 35, 1671 (1975)

  12. 12.

    Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265 (1951)

  13. 13.

    Lyon, M. F., Hawkes, S. G.: X-linked testicular feminization in the mouse. Nature (London) 227, 1217 (1970)

  14. 14.

    Morris, J. M., Mahesh, V. B.: Further observations on the syndrome “testicular feminization”. American Journal of Obstetrics and Gynecology 87, 731 (1963)

  15. 15.

    Neville, A. M., Engel, L. L.: The 32-1 dehydrogenase of bovine adrenal microsome. Endocrinology 83, 864 (1968)

  16. 16.

    Shikita, M., Tamaoki, B.: Testosterone formation by subcellular particles of rat testes. Endocrinology 76, 563 (1965)

  17. 17.

    Vanha-Perttula, T., Schneider, G., Bardin, C. W., Stanley, A. J., Gumbreck, L. G., Allison, J. E.: Testicular tumors in the androgen insensitive pseudohermaphrodite rat: Histochemistry and steroid metabolism. Gynecology Investigation 1, 149 (1970)

  18. 18.

    Wattenberg, L. W.: Microscopic histochemical demonstration of steroid-3β-OL dehydrogenase in tissue sections. Journal of Histochemistry and Cytochemistry 6, 225 (1958)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chung, K.W. Effects of LH and oestrogen on the growth of feminising testicular tumours in mice. Urol. Res. 9, 29–32 (1981).

Download citation

Key words

  • Testicular feminisation (tfm/y)
  • Testicular tumour
  • LH-Oestrogen