Advertisement

European Journal of Nuclear Medicine

, Volume 3, Issue 3, pp 191–195 | Cite as

Preparation and purification of estriol, estradiol, and progesterone labeled with radioactive iodine

  • A. Varvarigou
  • M. Villa
  • S. Rovano
Article

Abstract

Steroids labeled with radioactive isotopes are useful tracers for radio-immunoassay and for in vivo nuclear medecine purposes.

We here report and discuss methods for direct labeling of the steroids estriol, estradiol, and progesterone with radioiodine-125 or-131.

The purification procedure and some chemical and physical properties of the purified labeled materials are referred to and discussed.

Keywords

Public Health Purification Steroid Iodine Radioactive Isotope 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barton, G.M.R., Evans, R.S., Gardner, Y.A.: Paper chromatography of phenolic substances. Nature 170, 249 (1952)Google Scholar
  2. Comoglio, S., Galbiati, A., Rovano, S., Malvano, R.: L'impiego di traccianti iodati (125I) nel dosaggio radioimmunologico di steroidi. Giorn. It. Chim. Cl., 117–130 (1976)Google Scholar
  3. Ghanadian, R., Waters, S.L., Thakur, M.L., Chisholm, G.D.: Studies with radioactive iodine labelled oestrogens as prostate scanning agents. Int. J. Appl. Radiat. Isot. 26, 343–344 (1975)Google Scholar
  4. Ghanadian, R., Waters, S.L., Williams, G., Chisholm, G.A.: The use of 123I labelled oestradiol in detecting the human prostate using a gamma camera. Eur. J. Nucl. Med. 1, 159–160 (1976)Google Scholar
  5. Greenwood, F.C., Hunter, W.M., Glover, J.S.: The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochem. J. 84, 114 (1963)Google Scholar
  6. Helmkamp, R.W., Contreras, M.A., Bale, W.F.: I131-labeling of proteins by the iodine monochloride method. Int. J. Appl. Radiat. Isot. 18, 737–746 (1967)Google Scholar
  7. Hunter, W.M., Nars, P.W., Rutherford, F.J.: Preparation and behavior of 125I-labelled radioligands for phenolic and neutral steroids. Personal Communication (1973)Google Scholar
  8. Marchalonis, J.J.: Enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem. J. 113, 299 (1969)Google Scholar
  9. Midgley, A.R., Niswender, G.R.: Radioimmunoassay of steroid. Acta Endocrinol. [Suppl.] (Kbh.) 147, 64, 320 (1970)Google Scholar
  10. Neher, R.: Thin layer chromatography. A laboratory handbook, pp. 349–351 (1969)Google Scholar
  11. Shida, K., Shimazaki, J., Kurihara, H., Ito, Y., Furuya, N.: Scintiscanning of the prostatic tumors with 131I-labeled sex hormones. Proceedings of the 1o World Congress of Nucl. Med. 1974Google Scholar
  12. Sturman, M.F., Beierwaltes, W.H., Ice, R.D., Prakash, S.: Uptake of radiolabeled estradiol by the canine adrenal. J. Nucl. Med. 16, 1, 77–79 (1975)Google Scholar
  13. Thakur, M.L., Waters, S.L.: Evaluation of a method for the preparation of high specific activity radioiodinated oestradiol. Int. J. Appl. Radiat. Isot. 27, 585 (1976)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • A. Varvarigou
    • 1
    • 2
  • M. Villa
    • 3
    • 4
  • S. Rovano
    • 3
    • 4
  1. 1.Radiopharmaceutical Lab. N.R.C. DemocritosAthensGreece
  2. 2.A.E.A. Fellow (SC/202/GRE/7203) in Sorin BiomedicaSaluggiaItaly
  3. 3.Laboratorio di Fisiological Clinica del CNRPisaItaly
  4. 4.Sorin BiomedicaSaluggiaItaly

Personalised recommendations