Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

In-vivo and ex-vivo renal preservation

preliminary observations

  • 22 Accesses

  • 1 Citations


A new renal perfusate of modified intracellular electrolyte composition made hyperosmolar with mannitol and requiring no additional additives was successfully used to preserve canine kidneys “ex-vivo” for 48 hours by initial perfusion and hypothermic storage. The new perfusate was also successful in protecting totally ischemic canine kidneys from the lethal effect of two-hours of normothermic exposure “in-vivo”. — The preservation technique is uncomplicated, the materials involved are inexpensive, and the preservation apparatus is readily transportable.

This is a preview of subscription content, log in to check access.


  1. 1.

    Aquatella, H., Perez-Gonzalez, M., Morales, J. M., Whittenburg, G.: Ionic and histological changes in the kidney after perfusion and storage for transplantation. Transplantation 14, 480 (1972).

  2. 2.

    Belzer, F. O., Ashby, B. S., Dunphy, J. R.: 24-hour and 72-hour preservation of canine kidneys. Lancet 1967 II, 536.

  3. 3.

    Birkeland, S., Vogt, A., Krog, J., Semb, C.: Renal circulatory occlusion and local cooling. J. appl. Physiol. 14, 227 (1959).

  4. 4.

    Collins, G. M., Bravo-Shugarman, M., Terasaki, P. I.: Kidney preservation for transplantation. Lancet 1969 II, 1219.

  5. 5.

    Collins, G. M., Bravo-Shugarman, M., Terasaki, P. I.: Kidney preservation for transplantation. Transplantation 8, 821 (1969).

  6. 6.

    Edelman, I. S., Liebman, J.: Anatomy of body water and electrolytes. Amer. J. Med. 27, 256 (1957).

  7. 7.

    Flores, J., Dibona, D. R., Beck, C. H., Leaf, A.: The role of cell swelling in ischemic renal damage and the protective effect of hypertonic solute. J. clin. Invest. 51, 118 (1972).

  8. 8.

    Gazitua, S., Scott, J. R., Chow, C. C., Haddy, F. D.: Effect of osmolarity on canine renal vascular resistance. Amer. J. Physiol. 217, 1216 (1969).

  9. 9.

    Keeler, R., Swinney, J., Taytor, R. M. R., Uldall, P. R.: The problem of renal preservation. Brit. J. Urol. 38, 653 (1966).

  10. 10.

    Leaf, A.: Maintenance of concentration gradients and regulation of cell volume. Ann. N. Y. Acad. Sci. 72, 396 (1959).

  11. 11.

    Leaf, A.: Regulation of intracellular fluid volume and disease. Amer. J. Med. 49, 291 (1970).

  12. 12.

    Newburgh, L. H., Leaf, A.: Significance of body fluids in clinical medicine. Springfield, Illinois: Thomas 1955.

  13. 13.

    Nosowsky, E. E., Kaufman, J. J.: The protective action of mannitol in renal artery occlusion. J. Urol. 89, 295 (1963).

  14. 14.

    Sheehan, H. L., Davis, J. C., Renal ischemia with failed reflow. J. Path. Bact. 78, 105 (1959).

  15. 15.

    Summers, W. K., Jamison, R. L.: The no reflow phenomenon in renal ischemia. Lab. Invest. 25, 635 (1971).

Download references

Author information

Additional information

Supported by the Max Kade Foundation, New York, New York.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sacks, S.A., Petritsch, P.H., Battenberg, J.D. et al. In-vivo and ex-vivo renal preservation. Urol. Res. 1, 83–87 (1973).

Download citation

Key words

  • Renal preservation
  • renal transplantation
  • hypothermia
  • normothermia
  • hyperosmolar intracellular electrolyte solution