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Production of Bacillus sphaericus larvicide on industrial peptones

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A range of industrial peptones in combination with other carbon and nitrogen sources were investigated in order to define a medium for production of insecticide from Bacillus sphaericus, strain 2362. Industrial protein hydrolysates with an average peptide chain length above 30 were found to be poor substrates. Fermentation of B. sphaericus on protein-based media supplemented with glycerol resulted in production of 4–5x103 units of larvicide per ml fermentation broth, and toxicity of the larvicide up to 104 units per mg. Equally high larvicide production was obtained, when peptone was substituted with 30 mM l-glutamate and 2 mM proline. Addition of 2 mM l-arginine to this medium prevented sporulation and larvicide production.

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  1. de Barjac H, Veron M, Cosmao-Dumanoir V (1980) Caracterisation biochimique et serologique de souches de Bacillus sphaericus pathogenes ou non pour les moustiques. Ann Microbiol 131B:191–201

  2. Bourgouin C, Larget-Thiery I, de Barjac H (1984) Efficacy of dry powders from Bacillus sphaericus: RB 80 a potent reference preparation for biological titration. J Invertebr Pathol 44:146–150

  3. Broadwell AH, Baumann P (1986) Sporulation-associated activation of Bacillus sphaericus larvicide. Appl Environ Microbiol 52:758–764

  4. Davidson EW, Urbina M, Payne J, Mulla MS, Darwazeh H, Dulmage HT, Correa JA (1984) Fate of Bacillus sphaericus 1593 and 2362 spores used as larvicides in the aquatic environment. Appl Environ Microbiol 47:125–129

  5. Davidson EW (1985) Bacillus sphaericus as a microbial control agent for mosquito larvae. In: Laird M Miles J (eds). Mosquito control methodologies, vol. 2. Academic Press, London, pp 213–226

  6. Krych V, Johnson JL, Yousten AA (1980) Deoxyribo-nucleic acid homologies among strains of Bacillus sphaericus. Int J Syst Bacteriol 30:476–484

  7. Lacey LA (1984) Production and formulation of Bacillus sphaericus. Mosq News 44:153–159

  8. Lambert M, Neish AC (1950) Rapid method for estimation of glycerol in fermentation solutions. Can J Res 28:83–89

  9. Nicolas L, Dossou-Yovo J, Hougard JM (1987) Persistence and recycling of Bacillus sphaericus 2362 spores in Culex quinquefasciatus breeding sites in east Africa. Appl Microbiol Biotechnol 25:341–345

  10. Singer S (1980) Bacillus sphaericus for the control of mosquitoes. Biotechnol Bioeng 22:1335–1355

  11. Singer S, Goodman NS, Rogoff MH (1966) Defined media for the study of bacilli pathogenic to insects. Ann NY Acad Sci 139:16–23

  12. Weiser J (1984) A mosquito-virulent Bacillus sphaericus in adult Simulium damnosum from northern Nigeria. Z Mikrobiol 139:57–60

  13. White PJ, Lotay HK (1980) Minimal nutritional requirements of Bacillus sphaericus NCTC 9602 and 26 other strains of this species. J Gen Microbiol 118:13–19

  14. Yousten AA (1984) Bacillus sphaericus: Microbiological factors related to its potential as mosquito larvicide. Adv Biotechnol Proc 3:315–343

  15. Yousten AA, Wallis DA (1987) Batch and continuous culture production of the mosquito larval toxin of Bacillus sphaericus 2362. J Ind Microbiol 2:277–283

  16. Yousten AA, Madhekar N, Wallis DA (1984a) Fermentation conditions affecting growth sporulation and mosquito larval toxin formation by Bacillus sphaericus. Dev Ind Microbiol 25:757–762

  17. Yousten AA, Wallis DA, Singer S (1984b) Effect of oxygen on growth, sporulation and mosquito larval toxin formation by Bacillus sphaericus 1593. Curr Microbiol 11:175–178

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Correspondence to S. Braun.

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Klein, D., Yanai, P., Hofstein, R. et al. Production of Bacillus sphaericus larvicide on industrial peptones. Appl Microbiol Biotechnol 30, 580–584 (1989). https://doi.org/10.1007/BF00255363

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  • Peptide
  • Toxicity
  • Fermentation
  • Glycerol
  • Proline