The activities of mixed leucocyte cultures (MLC) prepared from pairs of healthy subjects were estimated from the uptake of tritiated thymidine by the cultures. It was confirmed that MLC is a practical method for detecting identical twins amongst siblings. MLC has not often been employed for donor selection for renal transplantation, mainly because of the impaired MLC reactivity of leukocytes from uraemic patients. An attempt was made to separate lymphocytes from uraemic leukocyte-rich plasma, which always has a high proportion of neutrophils. A lymphocyterich fraction of uraemic leukocytes has MLC reactivity comparable to that of non-uraemic leukocytes.
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Bain, B., Vas, M. R., Lowenstein, L.: The development of large immature mononuclear cells in mixed leukocyte cultures. Blood 23, 108 (1964).
Dutton, R. W.: Spleen cell proliferation in response to homologous antigens studied in congenic resistant strains of mice. J. exp. Med. 123, 665 (1966).
Wilson, D. B.: Quantitative studies on the mixed lymphocyte interaction in rats. J. exp. Med. 126, 625 (1967).
Bach, F. H., Kisken, W. A.: Predictive value of results of mixed leukocyte cultures for skin allograft survival in man. Transpl. 5, 1046 (1967).
Kasakura, S., Lowenstein, L.: Irradiated and preserved leukocytes in mixed leukocyte cultures. Proc. Soc. exp. Biol. Med. 125, 355 (1967).
Bach, F. H., Voynow, N. Y.: One-way stimulation in mixed leukocyte cultures. Science 153, 545 (1966).
Bach, F. H., Amos, D. B.: Hu-l: major histocompatibility locus in man. Science 156, 1508 (1967).
Singal, D. P., Mickey, M. R., Terasaki, P. I.: Serotyping for homotransplantation. Transplantation 7, 246 (1969).
Kasakura, S., Lowenstein, L.: The effect of uremic blood on mixed leukocyte reactions and on cultures of leukocytes with phytohemagglutinin. Transplantation 5, 283 (1967).
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Kojima, H. Mixed leukocyte culture in uraemia. Urol. Res. 1, 11–14 (1973). https://doi.org/10.1007/BF00254758
- Mixed leukocyte culture
- histocompatibility test
- uraemid subjects