Human prostatic tissue and expressed prostatic secretions (EPS) efrom patients with benign prostatic hyperplasia (BPH) and prostate cancer were incubated with (1 β 3H) androstenedione. The extent of aromatization was determined by measuring the transfer of 3H from the 1 β position into water. The amount of 3H2O recovered corresponds to the estrogens formed. Tissue from 5 patients with BPH yielded 2.13 (±1.05) pmol/mg protein/h while the EPS from the same patients yielded 727 fmol/mg protein/h. In patients with prostate cancer the mean formation of estrogens was 388 fmol/mg protein/h (±75). 4-hydroxyandrostenedione, an aromatase inhibitor, successfully inhibited aromatization in BPH and prostate cancer 53–98%.
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Anderson RU, Fair WR (1976) Physical and chemical determinations of prostatic secretion in benign hyperplasia, prostatitis, and adenocarcinoma. Invest Urol 14:137–139
Bouton MM, Porin C, Grandadan JA (1981) Estrogen regulation of rat prostate androgen receptor. J Steroid Biochem 15:403–408
Cooms RC, Dowsett M, Goss P, Gazet JC, Brodie A (1984) 4-hydroxyandrostenedione in treatment of postmenopausal patients with advanced breast cancer. Lancet 1237–1239
Fishman J, Goto J (1981) Mechanisms of estrogen biosynthesis. J Biol Chem 256:4466–4471
Grayhack JT, Lee C, Oliver L, Schaeffer AJ, Wendel EF (1980) Bichemical profiles of prostatic fluid from normal and diseased prostate glands. Prostate 1:227–237
Harper ME, Pierrepoint CG, Griffiths K (1984) Carcinoma of the prostate: relationship of pretreatment hormone levels to survival. Eur J Cancer Clin Oncol 20:477–482
Kozak I, Bartsch W, Krieg M, Voigt KD (1982) Nuclei of stroma: site of highest estrogen concentration in human prostatic hyperplasia. Prostate 3:433–438
Krieg M, Bartsch W, Thompson M, Voigt KD (1983) Androgens and estrogens: their interaction with stroma and epithelium of human benign prostatic hyperplasia and normal prostate. J Steroid Biochem 19:155–161
Lowry OH, Rosenburgh NJ, Farr LA, Randall RJ (1951) Protein measurement with the folin penol reagent. J Biol Chem 193:265–269
Meikle AW, Stanish WM (1982) Familial prostatic cancer risk and low testosterone. J Clin Endocrinol Metab 54:1104–1107
Miyairi S, Fishman J (1985) Radiometric analysis of oxidative reactions in aromatization by placental microsomes. J Biol Chem 260:320–325
Rose DR, Laakso K, Sotarauta M, Wynder EL (1984) Hormone levels in prostatic fluid from healthy finns and prostate cancer patients. Eur J Cancer Clin Oncol 10:1317–1319
Schweikert HU (1979) conversion of androstenedione to estrone in human fibroblasts cultured from prostate, genitals and nongenital skin. Hormone Metab Res 11:635–640
Stone NN, Fair WR, Fishman J (1986) Estrogen formation in human prostatic tissue from patients with and without benign prostatic hyperplasia. Prostate 9:311–318
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Stone, N.N., Laudone, V.P., Fair, W.R. et al. Aromatization of androstenedione to estrogen by benign prostatic hyperplasia, prostate cancer and expressed prostatic secretions. Urol. Res. 15, 165–167 (1987). https://doi.org/10.1007/BF00254430
- Prostate cancer
- Prostatic secretions