Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Regional coupling of blood flow and methionine uptake in an experimental tumor assessed with autoradiography

  • 29 Accesses

  • 2 Citations

Abstract

Regional distribution of L-[methyl-14C] methionine (14C-MET) and 4-[18F] fluoro-antipyrine was compared using experimental rat tumors (AH109A) and a computerized autoradiogram image processor. Tissue distributions of the two tracers were found to be inhomogeneous in the tumor with nearly identical image patterns. Analysis of tissue radioactivities revealed that 82% of 14C-MET was derived from the acid insoluble fraction at 60 min after injection. The present study showed that 14C-MET uptake closely relates to tissue blood flow and may depend on its blood to tissue transport. Rapid incorporation of MET in the acid insoluble fraction implies that it is rapidly metabolized after transport into tumor tissue.

This is a preview of subscription content, log in to check access.

References

  1. Ashe H, Clark BR, Chu F, Hardy DN, Halpern BC, Halpern RM, Smith RA (1974) N5-methyltetrahydrofolate: Homocysteine methyltransferase activity in extracts from normal, malignant and embryonic tissue culture cells. Biochem Biophys Res Commun 57:417–425

  2. Bergstrom M, Collins VP, Ehrin E, Ericson K, Eriksson L, Greit T, Halldin C, von Holst H, Langstrom B, Lilja A, Lundquist H, Nagren K (1983) Discrepancies in brain tumor extent as shown by computed tomography and positron emission tomography using 68Ga-EDTA, 11C-glucose, and 11C-methionine. J Comput Assist Tomogr 7:1062–1066

  3. Comar D, Cartron JC, Maziere M, Marazano C (1976) Labelling and metabolism of methionine-methyl-11C. Eur J Nucl Med 1:11–14

  4. Edlich RF, Tookenay V (1969) Tumor blood flow and its distribution. Arch Surg 98:111–114

  5. Ericson K, Lilja A, Bergstrom M, Collin VP, Eriksson L, Ehrin E, von Host H, Lundquist H, Langstrom B, Mosskin M (1985) Positron emission tomography with 11C-methyl-L-methionone, 11C-D-glucose, and 68Ga-EDTA in supratentorial tumors. J Comput Assist Tomogr 9:683–689

  6. Gullino PM, Graham FH (1961) Studies on the exchange of fluid between host and tumor. J Natl Cancer Inst 27:1465–1491

  7. Halpern BC, Clark BR, Hardy DN, Halpern RN, Smith RA (1974) The effect of replacement of methionine by homocysteine on survival of malignant and normal adult mammalian cells in culture. Proc Natl Acad Sci USA 71:1133–1136

  8. Hoffman RF, Erbe RW (1976) High in vitro rates of methionine biosynthesis in transformed human and malignant rat cells auxotrophic for methionine. Proc Natl Acad Sci USA 73:1523–1527

  9. Ishiwata K, Ido T, Abe Y, Matsuzawa T, Murakami M (1985) Studies on 18F-labeled pyrimidines III. Biochemical investigation of 18F-labeled pyrimidines and comparison with 3H-deoxythymidine in tumor-bearing rats and mice. Eur J Nucl Med 10:39–44

  10. Jain RK, Weissbrod JM, Wei J (1980) Mass transport in tumors: characterization and applications to chemotherapy. Adv Cancer Res 33:251–310

  11. Kairento AL, Brownell GL, Schluederberg J, Elmalch DR (1983) Regional blood flow measurement in rabbit soft-tissue tumor with positron imaging using the C15O2 steady state and labeled microspheres. J Nucl Med 24:1135–1142

  12. Kreis W, Goodenow M (1978) Methionine requirement and replacement by homocysteine in tissue cultures of selected rodent and human malignant and normal cells. Cancer Res 38:2259–2262

  13. Kubota K, Itoh M, Fukuda H, Abe Y, Fujiwara T, Hatazawa J, Iwata R, Ishiwata K, Yoshioka S, Matsuzawa T, Watanuki S, Ido T (1983) Cancer diagnosis with positron computed tomography and carbon-11-labeled L-methionine. Lancet ii:1192

  14. Kubota K, Matsuzawa T, Itoh M, Itoh K, Fujiwara T, Abe Y, Yoshioka S, Hatazawa J, Watanuki S, Ido T (1985) Lung tumor imaging by positron emission tomography using C-11 L-methionine. J Nucl Med 26:37–42

  15. Lear JL, Ackermann R, Kameyama M, Carson R, Phelps M (1984) Multiple-radionuclide autoradiography in evaluation of cerebral function. J Cereb Blood Flow Metab 4:264–269

  16. Meyer GJ, Schober O, Hundeshagen H (1985) Uptake of 11C-L-and D-methionine in brain tumors. Eur J Nucl Med 10:373–376

  17. Mies G, Niebuhr I, Hossman K (1981) Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques. Stroke 12:581–588

  18. Rodgers W, Edlich RF, Lewis DV, Aust JB (1967) Tumor blood flow I. Blood flow in transplantable tumors during growth. Surg Clin North Am 47:1473–1482

  19. Sako K, Diksic M, Kato A, Yamamoto YL, Feindel W (1984) Evaluation of 18F-4-fluoro-antipyrine as a new blood flow tracer for multiradionuclide autoradiography. J Cereb Blood Flow Metab 4:259–263

  20. Shiue CY, Wolf AP (1981) Synthesis of 4-fluoro-2,3-dimethyl-1-phenyl-3-pyrazoline-5-one(4-fluoroantipyrine) and 18F-labeled analog by direct fluorination of antipyrine with molecular fluorine. J Label Compds Radiopharm 18:1059–1066

  21. Straw JA, Hart MM, Klubes P, Zaharko DS, Dedrick RL (1974) Distribution of anti-cancer agents in spontaneous animal tumors. 1. Regional blood flow and methotrexate distribution in canine lymphosarcoma. J Natl Cancer Inst 52:1327–1331

  22. Tisdale MJ (1980a) Changes in tRNA methyltransferans activity and cellular S-adenosylmethionine content following methionine deprivation. Biochim Biophys Acta 609:296–305

  23. Tisdale MJ (1980b) Effect of methionine deprivation on methylation and synthesis of macromolecules. Br J Cancer 42:121–128

  24. Wilson MJ, Poirier LA (1978) An increased requirement for methionine by transformed rat liver epithelial cells in vitro. Exp Cell Res 111:397–400

Download references

Author information

Correspondence to Taiju Matsuzawa.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Abe, Y., Matsuzawa, T., Itoh, M. et al. Regional coupling of blood flow and methionine uptake in an experimental tumor assessed with autoradiography. Eur J Nucl Med 14, 388–392 (1988). https://doi.org/10.1007/BF00254390

Download citation

Key words

  • Blood flow
  • Methionine uptake
  • Rat Tumor
  • Autoradiography