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In vivo labelling of granulocytes with 99mTc anti-NCA monoclonal antibodies for imaging inflammation

Abstract

We looked for inflammatory lesions in 45 patients using in vivo labelled granulocytes. For the purposes of cell labelling we used a murine, monoclonal antibody reacting with NCA and CEA (BW 250/183) (Bosslet et al. 1985) labelled with 99mTc-pertechnetate. All abscesses and other inflammatory lesions were visualized with excellent quality scintigrams between 2 and 6 h after the injection. As the antibody can be stored in a freeze-dried form and labelled at any time in any Department of Nuclear Medicine with 99mTc-pertechnetate, without cell isolation being necessary, the method appears to be suitable even for use in acute diagnosis. No side-effects have so far occurred, even in patients injected up to three times.

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Correspondence to K. Joseph.

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Joseph, K., Höffken, H., Bosslet, K. et al. In vivo labelling of granulocytes with 99mTc anti-NCA monoclonal antibodies for imaging inflammation. Eur J Nucl Med 14, 367–373 (1988). https://doi.org/10.1007/BF00254386

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Key words

  • Monoclonal antibody
  • 99mTechnetium
  • Inflammation in vivo labelling
  • Immunoscintigraphy