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European Biophysics Journal

, Volume 16, Issue 5, pp 313–319 | Cite as

Galvanotaxis of human granulocytes

Dose-response curve
  • B. Rapp
  • A. de Boisfleury-Chevance
  • H. Gruler
Article

Abstract

The galvanotactic response of human granulocytes was investigated theoretically and experimentally. The basic results are: (i) The granulocytes move towards the anode. (ii) The directed movement has been quantified by two different polar order parameters-the McCutcheon index and the average of cos ϕ. (iii) The polar order parameters are a function of the applied electric field (= dose-response curve). (iv) The inverse of the galvanotactic constant of migrating cells (analogous to the Michaelis-Menten constant) has a value of-0.2±0.03 V/mm. (v) The galvanotactic response of granulocytes is a non-cooperative process with a cooperativity coefficient of 1±0.2. (vi) The galvanotactic constant is a function of pH. (vii) The protein essential for the galvanotactic response is very likely a G-protein.

Key words

Galvanotaxis granulocytes dose-response curve 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • B. Rapp
    • 1
  • A. de Boisfleury-Chevance
    • 2
  • H. Gruler
    • 1
  1. 1.Abteilung für BiophysikUniversität UlmUlmFederal Republic of Germany
  2. 2.Centre d'Etudes sur l'Ecologie des Cellules du Sang, Département d'HématologieHôpital de la SalpétrièreParisFrance

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