Callus culture was initiated from expiants of mature root tissues of ginseng (Panax ginseng C.A. Meyer) on MS medium enriched with 2,4-D. The ageing callus produced numerous embryoids in this medium. Reculture of these embryoids in media (1/2 MS or B5) supplemented with benzyladenine and gibberellic acid resulted in profuse plantlet regeneration.
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Butenko, R., Brushwitzky, I.V., Slepyan, L.I. (1968): Organogenesis and somatic embryogenesis in the tissue culture of Panax ginseng C.A. Meyer. Bot. Zh. 7, 906–911
Chang, W.C., Hsing, Y.I. (1978a): Callus induction, growth and rhizogenesis from roots of Panax ginseng C.A. Meyer. Natl. Sci. Council Monthly (ROC) 6, 76–79
Chang, W.C., Hsing, Y.I. (1978b): Somatic embryogenesis of root callus of Panax ginseng C.A. Meyer on a defined medium. Natl. Sci. Council Monthly (ROC) 6, 770–772
Chang, W.C., Hsing, Y.I. (1978c): Shoot formation on root callus of Panax ginseng C.A, Meyer in vitro. Natl. Sci. Council Monthly (ROC) 6, 1171–1173
Gamborg, O.L., Miller, R.A., Ojima, K. (1968): Nutrient requirements of suspension culture of soybean root callus. Exp. Cell. Res. 50, 151–158
Jhang, J.J., Staba, E.J., Kim, J.Y. (1974): American and Korean ginseng tissue cultures: growth, chemical analysis, and plantlet production. In Vitro 9, 253–259
Murashige, T., Skoog, F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–479
Communicated by G. Melchers
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Chang, W.C., Hsing, Y.I. Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C. A. Meyer). Theoret. Appl. Genetics 57, 133–135 (1980). https://doi.org/10.1007/BF00253888
- Somatic embryogenesis
- Root callus
- Plantlet regeneration
- Panax ginseng