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Radiolabeled DNase, a potential indicator for noninvasive detection of tissue damage

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Abstract

Pancreatic DNase I was labeled with 131I or fluorescamine and injected IV into NMRI mice bearing a sarcoma 180. Of the injected tracer, 1.5%–2% was found to accumulate per g tumor. In sections of tumor tissue DNase was localized in damaged cells in solid and necrotic tumor regions. This binding is most probably due to specific interaction of DNase with actin, an ubiquitous cytoskeletal protein. Two-component blood clearance with a rapid first component (two–thirds of applied radioactivity) was observed. The labeled tumor could easily be visualized by gamma camera imaging. The findings suggest DNase to be a potent radiopharmaceutical for imaging damaged tissue, occurring in malignant tumors as well as in infarcts, and inflammatory lesions.

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References

  1. 1.

    Anderson PJ (1976) The actin of muscle and fibroblasts. Biochem J 155:297–301

  2. 2.

    Bray D, Thomas C (1975) The actin content of fibroblasts. Biochem J 147:221–229

  3. 3.

    Coates G, DeNardo J, DeNardo GL, Troy FA (1975) Pharmacokinetics of radioiodinated streptokinase. J Nucl Med 16:136–142

  4. 4.

    Day ED (1964) Vascular relationships of tumors and host. Prog Exp Tumor Res 4:57–97

  5. 5.

    Dunham LJ, Schlyen SM (1959) Transplantable and transmissible tumors of animals. Armed Forces Inst Pathol 40, Natl Acad Sci Natl Res Counc Washington DC, 1959

  6. 6.

    Greenwood FC, Hunter MW, Glover JS (1963) The preparation of 131I-labeled human growth hormone of high specific radioactivity. Biochem J 89:114–123

  7. 7.

    Hitchcock SE, Carlsson L, Lindberg U (1976) Depolymerization of F-Actin by deoxyribonuclease I. Cell 7:531–542

  8. 8.

    Laemmli K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

  9. 9.

    Machulla HJ, Marsmann M, Dutschka K (1980) Biochemical concept and synthesis of a radioiodinated phenyl fatty acid for in vivo metabolic studies of the myocardium. Eur J Nucl Med 5:171–173

  10. 10.

    Pollard TD, Weihing RR (1974) Actin and myosin and cell movement. CRC Crit Rev biochem 1–65

  11. 11.

    Reske SN, Vyska K, Reske K, Feinendegen LE (1981) External detection of myocardial cell injury. J Nucl Med 22:6, P 73, (abstr)

  12. 12.

    Udenfried S, Stein S, Boehlen P (1972) Fluorescamine: A reagent for assay of amino acids, peptides, proteins and primary Amines in the picomole range. Science 178:971–972

  13. 13.

    Winchell HS (1976) Mechanisms for localization of radiopharmaceuticals in neoplasms. Semin Nucl Med 6:371–378

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Correspondence to S. N. Reske.

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Reske, S.N., Reske, K. & Winkler, C. Radiolabeled DNase, a potential indicator for noninvasive detection of tissue damage. Eur J Nucl Med 8, 284–287 (1983). https://doi.org/10.1007/BF00253752

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Keywords

  • Sarcoma
  • Damage Cell
  • Specific Interaction
  • Gamma Camera
  • Tumor Region