Conditions are described for converting bagasse lignocellulose to volatile fatty acids (VFA) by anaerobic fermentation. Although yields of VFA were as high as 74% by weight of digestible organic matter (or 54% of dry bagasse), limitations were imposed by both fermenter design and fibre digestibility. All fermentations were substrate-limited up to the maximum initial concentration examined of 50 g bagasse · l-1 and no product inhibition was evident (up to 260 mM VFA produced). Maximum VFA productivities of 0.25 to 0.65 g · l-1 · h-1 were obtained in batch fermentations and this is greater than those previously reported using lignocellulosic substrates. Batch fermentations neared completion after 66 h.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Britz ML, Wilkinson RG (1979) The isolation and properties of metronidazole-resistant mutants of Bacteriodes fragilis. Antimicrob Agents Chemother 16: 19–27
Bryant MP, Burkey LA (1953) Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J Dairy Sci 36: 205–217
Datta R (1981) Acidogenic fermentation of corn stover. Biotechnol Bioeng 23: 61–77
Depasse E (1945) Vue d'ensemble d'une production industrielle de cétones. Bull Assoc Chim Sucr Distill Fr 62: 317–339
Goering HK, van Soest PJ (1970) Forage fiber analyses. USDA Agriculture Handbook No 379
Holdeman LV, Cato EP, Moore WEC (1977) Anaerobe laboratory manual, 4th edn. Anaerobe Laboratory, Virginia Polytechnic Inst, State University, Blacksburg, Virginia
Langwell H (1932) Cellulose fermentation. Chem Ind 51: 988–994
Leedle JAZ, Hespell RB (1980) Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations. Appl Environ Microbiol 39: 709–719
McLeod MN, Minson DJ (1978) The accuracy of the pepsin-cellulase technique for estimating the dry matter digestibility in vivo of grasses and legumes. Anim Feed Sci Technol 3: 277–287
Minato H, Suto T (1978) Technique for fractionation of bacteria in rumen microbial ecosystem. II. Attachment of bacteria isolated from bovine rumen to cellulose powder in vivo and elution of bacteria attached therefrom. J Gen Appl Microbiol 24: 1–16
Péaud-Len oel C (1952) La production d'acides gras volatils par fermentation. Ind Agric Aliment 69: 211–220
Playne MJ (1980) Microbial conversion of cereal straw and bran into volatile fatty acids—key intermediates in the production of liquid fuels. Food Technol Aust 32: 451–456
Playne MJ (1981) Volatile fatty acid production by anaerobic fermentation of ligno-cellulosic substrates. In: Advances in biotechnology, vol 2. Proc 6th Internat Fermentation Symp (London Canada July 1980). Pergamon Press, New York, pp 85–90
Playne MJ (1984) Increased digestibility of bagasse by pretreatment with alkalis and steam explosion. Biotechnol Bioeng 26: 426–433
Sanderson JE, Wise DL, Augenstein DC (1978) Organic chemicals and liquid fuels from algal biomass. Biotechnol Bioeng Symp 8: 131–151
Schwartz RD, Keller FA, Jr (1982) Isolation of a strain of Clostridium thermoaceticum capable of growth and acetic acid production at pH 4.5 Appl Environ Microbiol 43: 117–123
Smith BR, Playne MJ (1982) Recovery of volatile fatty acids from fermenter effluents and their conversion to liquid fuels. Proc 1st ASEAN Workshop on Fermentation Technology, Kuala Lumpur (ASEAN Committee on Science and Technology), pp 510–524
Theuninck DH, Goodrich RD, Meiske JC (1981) Influence of Captan on in vitro and in vivo digestibility of forage. J Anim Sci 52: 377–381
Torre I de la, Goma G (1981) Characterization of anaerobic microbial culture with high acidogenic activity. Biotechnol Bioeng 23: 185–199
About this article
Cite this article
Matei, C.H., Playne, M.J. Production of volatile fatty acids from bagasse by rumen bacteria. Appl Microbiol Biotechnol 20, 170–175 (1984). https://doi.org/10.1007/BF00253726
- Organic Matter
- Volatile Fatty Acid
- Batch Fermentation