Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Conversion of starch into ethanol by Clostridium thermohydrosulfuricum

  • 36 Accesses

  • 8 Citations

Summary

Production of ethanol from starch by Clostridium thermohydrosulfuricum was compared with that from glucose, fructose or maltose in batch fermentations. Optimal substrate concentration and pH for ethanol production were determined. The rate of ethanol production on starch was about the same as that on glucose or fructose and overall yields were also similar (about 1.6 mol ethanol per mol glucose or glucose equivalent). Maltose was not an effective substrate for growth and ethanol production.

When a mixture of starch and glucose in equal amounts was used, breakdown of starch and utilization of glucose were simultaneous. When starch and fructose were supplied together, the fructose was utilized but no hydrolysis of starch was observed. With a mixture of glucose and fructose, uptake of fructose preceeded that of glucose.

This is a preview of subscription content, log in to check access.

References

  1. Andreesen JR, Schaupp A, Neurauter C, Brown A, Ljungdahl LG (1973) Fermentation of glucose, fructose and xylose by Clostridium thermoaceticum: Effect of metals on growth yield, enzymes and the synthesis of acetate from CO2. J Bacteriol 114:743–751

  2. Ben-Bassat A, Lamed R, Zeikus JG (1981) Ethanol production by thermophilic bacteria: Metabolic control of end product formation in Thermoanaerobium brockii. J Bacteriol 146:192–199

  3. Ben-Bassat A, Zcikus JG (1981) Thermobacteroides acetoethylicus gen nov and spec nov, a new chemoorganotrophic, anaerobic, thermophilic bacterium. Arch Microbiol 128:365–370

  4. Carreira LH, Wiegel J, Ljungdahl LG (1983) Production of ethanol from biopolymers by anaerobic, thermophilic and extreme thermophilic bacteria: I Regulation of carbohydrate utilization in mutants of Thermoanaerobacter ethanolicus. Biotechnol Bioeng Symp 13:183–191

  5. Gutmann I (1974) Maltose. In: Bergmeyer HU (ed) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, pp 1152–1155

  6. Hansen SA (1975) Thin-layer chromatographic method for identification of oligosaccharides in starch hydrolyzates. J Chromatogr 105:388–390

  7. Harris P, Kornberg HL (1972) The uptake of glucose by a thermophilic Bacillus sp. Proc R Soc Lond B 182:159–170

  8. Huyn HH, Zeikus JG (1985a) General biochemical characterization of thermostable pullulanase and glucoamylase from Clostridium thermohydrosulfuricum. Appl Environ Microbiol 49:1168–1173

  9. Huyn HH, Zeikus JG (1985b) Simultaneous and enhanced production of thermostable amylases and ethanol from starch by cocultures of Clostridium thermosulfurogenes and Clostridium thermohydrosulfuricum. Appl Environ Microbiol 49:1174–1181

  10. Parkkinen E, Korhola M (1984) Starch fermentation by Clostridium thermohydrosulfuricum. In: European Federation of Biotechnology (ed) Third European Congress on Biotechnology, Verlag Chemie, Weinheim, vol II, pp 223–230

  11. Parkkinen E, Mattila A (1982) Die Vergärung verschiedener Zucker durch Clostridium thermohydrosulfuricum. In: Dellweg H (ed) 5 Symp Techn Mikrobiol, Berlin, pp 237–243

  12. Sills AM, Zygora PSJ, Stewart GG (1984) Characterization of Schwanniomyces castelli mutants with increased productivity of amylases. Appl Microbiol Biotechnol 20:124–128

  13. Srivastava RAK, Mathur SN (1984) Regulation of amylase bio-synthesis in growing and non-growing cells of Bacillus stearothermophilus. J Appl Bacteriol 57:147–151

  14. Taya M, Suzuki Y, Kobayashi T (1984) A thermophilic anaerob (Clostridium species) utilizing various biomass-derived carbohydrates. J Ferment Technol 62:229–236

  15. Wiegel J, Ljungdahl LG (1979) Ethanol as fermentation product of extreme thermophilic anaerobic bacteria. In: Dellweg H (ed) 4 Symp Techn Microbiol, Berlin, pp 117–127

  16. Wiegel J, Ljungdahl LG (1981) Thermoanaerobacter ethanolicus gen nov, spec nov, a new, extreme thermophilic, anaerobic bacterium. Arch Microbiol 128:343–348

  17. Wiegel J, Ljungdahl LG, Rawson JR (1979) Isolation from soil and properties of the extreme thermophile Clostridium thermohydrosulfuricum. J Bacteriol 139:800–810

  18. Zeikus JG, Hegge PW, Anderson MA (1979) Thermoanaerobium brockii gen nov and sp nov, a new chemoorganotrophic, caldoactive, anaerobic bacterium. Arch Microbiol 122:41–48

Download references

Author information

Correspondence to Elke Parkkinen.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Parkkinen, E. Conversion of starch into ethanol by Clostridium thermohydrosulfuricum . Appl Microbiol Biotechnol 25, 213–219 (1986). https://doi.org/10.1007/BF00253651

Download citation

Keywords

  • Glucose
  • Hydrolysis
  • Fermentation
  • Starch
  • Fructose